Annatto tocotrienol improves indices of bone static histomorphometry in osteoporosis due to testosterone deficiency in rats

Chin Kok Yong, Saif Abdul-Majeed, Nur Farhana Mohd Fozi, Ima Nirwana Soelaiman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This study aimed to evaluate the effects of annatto tocotrienol on indices of bone static histomorphometry in orchidectomized rats. Forty male rats were randomized into baseline (BL), sham (SH), orchidectomized (ORX), annatto tocotrienol-treated (AnTT) and testosterone enanthate-treated (TE) groups. The BL group was sacrificed upon receipt. All rats except the SH group underwent bilateral orchidectomy. Annatto tocotrienol at 60 mg/kg body weight was administered orally daily to the AnTT group for eight weeks. Testosterone enanthate at 7 mg/kg body weight was administered intramuscularly once weekly for eight weeks to the TE group. The rat femurs were collected for static histomorphometric analysis upon necropsy. The results indicated that the ORX group had significantly higher osteoclast surface and eroded surface, and significantly lower osteoblast surface, osteoid surface and osteoid volume compared to the SH group (p < 0.05). Annatto tocotrienol and testosterone enanthate intervention prevented all these changes (p < 0.05). The efficacy of annatto tocotrienol was on par with testosterone enanthate. In conclusion, annatto tocotrienol at 60 mg/kg can prevent the imbalance in bone remodeling caused by increased osteoclast and bone resorption, and decreased osteoblast and bone formation. This serves as a basis for the application of annatto tocotrienol in hypogonadal men as an antiosteoporotic agent.

Original languageEnglish
Article number23
Pages (from-to)4974-4983
Number of pages10
JournalNutrients
Volume6
Issue number11
DOIs
Publication statusPublished - 10 Nov 2014

Fingerprint

Tocotrienols
Bixa orellana
tocotrienols
osteoporosis
Osteoporosis
testosterone
Testosterone
bones
Bone and Bones
rats
osteoclasts
osteoblasts
Osteoclasts
Osteoblasts
Body Weight
bone resorption
body weight
Orchiectomy
annatto
Bone Remodeling

Keywords

  • Osteoblast
  • Osteoclast
  • Osteoporosis
  • Testosterone
  • Tocotrienol
  • Vitamin E

ASJC Scopus subject areas

  • Food Science

Cite this

Annatto tocotrienol improves indices of bone static histomorphometry in osteoporosis due to testosterone deficiency in rats. / Kok Yong, Chin; Abdul-Majeed, Saif; Fozi, Nur Farhana Mohd; Soelaiman, Ima Nirwana.

In: Nutrients, Vol. 6, No. 11, 23, 10.11.2014, p. 4974-4983.

Research output: Contribution to journalArticle

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