Angiotensin I-converting enzyme inhibitory peptides from snakehead fish sarcoplasmic protein hydrolysate

Masomeh Ghassem, Abd. Salam Babji, Mamot Said, Fatemeh Mahmoodani, Keizo Arihara

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this study, the angiotensin I-converting enzyme (ACE) inhibitory peptides were isolated from snakehead fish sarcoplasmic protein hydrolysates. Enzymatic hydrolysis of sarcoplasmic protein was performed using various commercial enzymes. The alcalase hydrolysate with the highest ACE inhibition activity was purified with gel chromatography and reversed phased high-performance liquid chromatography. The purified fractions were then subjected to electrospray ionization quadrupole-micro-time-of-flight mass spectrometry for amino acid characterization. Two novel ACE inhibitory peptides LYPPP and YSMYPP with IC50 values of 1.3 and 2.8μM were identified, respectively. The pattern of ACE inhibition, resistance to hydrolysis by gastrointestinal proteases and cytotoxic potencies of isolated peptides were described. The results showed no cytotoxicity of peptides on human embryonic fibroblast cell line (MRC-5) and human hepatocarcinoma cell line (HepG2).

Original languageEnglish
Pages (from-to)140-149
Number of pages10
JournalJournal of Food Biochemistry
Volume38
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Fish Proteins
Channidae
Protein Hydrolysates
protein hydrolysates
peptidyl-dipeptidase A
Peptidyl-Dipeptidase A
peptides
Peptides
enzyme inhibition
fish
Hydrolysis
Subtilisins
Cell Line
human cell lines
subtilisin
enzymatic hydrolysis
hydrolysates
ionization
Inhibitory Concentration 50
fibroblasts

ASJC Scopus subject areas

  • Food Science
  • Cell Biology
  • Pharmacology
  • Biophysics

Cite this

Angiotensin I-converting enzyme inhibitory peptides from snakehead fish sarcoplasmic protein hydrolysate. / Ghassem, Masomeh; Babji, Abd. Salam; Said, Mamot; Mahmoodani, Fatemeh; Arihara, Keizo.

In: Journal of Food Biochemistry, Vol. 38, No. 2, 2014, p. 140-149.

Research output: Contribution to journalArticle

Ghassem, Masomeh ; Babji, Abd. Salam ; Said, Mamot ; Mahmoodani, Fatemeh ; Arihara, Keizo. / Angiotensin I-converting enzyme inhibitory peptides from snakehead fish sarcoplasmic protein hydrolysate. In: Journal of Food Biochemistry. 2014 ; Vol. 38, No. 2. pp. 140-149.
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