Kaedah analatikal bagi penentuan monosakarida daripada serabut sawit terawat alkali

Translated title of the contribution: Analytical approaches of determining monosaccharides from alkaline-treated palm fiber

Khairiah Badri, Cheah Ai Juan, Osman Hassan, Wan Aida Wan Mustapha

Research output: Contribution to journalArticle

Abstract

Monosaccharides in oil palm empty fruit bunch fiber (EFB) were determined by methanolysis and acetylation. Three types of EFB samples, namely untreated EFB, EFB pretreated with hot water and EFB pretreated with hot water followed by 10% (w/w) sodium hydroxide (NaOH) aqueous solution were used. The FTIR spectrum indicated the disappearance and shifting of aromatic and carbonyl functional groups, syringyl propane unit, guaisacyl propane unit and C-H lignin. The filter cake undergone methanolysis and alditol acetate treatments to detect the composition of reducing sugars. Gas chromatography flame ionization detector (GC-FID) analysis was conducted to determine the type and quantity of reducing sugars produced. Acetylation produced two types of monosaccharides namely glucose and galactose whereas methanolysis detected only one type of monosaccharide, which was xylose. The extracted monosaccharides obtained from hot water pretreatment followed by 10% (w/w) NaOH aqueous solution treatment analysed by methanolysis and acetylation were 178.4 mg/g xylose and 29.9 mg/g glucose respectively. About 0.76 mg/g xylose was extracted from hot water pretreated EFB fiber by methanolysis. Acetylation detected monosaccharides in untreated EFB and identified as glucose with the amount of 19.15 mg/g, whereas monosaccharides from hot water pretreated EFB fiber were identified as glucose and galactose at 6.32 mg/g and 2.83 mg/g respectively.

Original languageUndefined/Unknown
Pages (from-to)46-54
Number of pages9
JournalMalaysian Journal of Analytical Sciences
Volume19
Issue number1
Publication statusPublished - 2015

Fingerprint

Monosaccharides
Fruits
Fibers
Acetylation
Xylose
Water
Glucose
Propane
Galactose
Sugars
Sugar Alcohols
Sodium Hydroxide
Palm oil
Lignin
Gas chromatography
Functional groups
Ionization
Acetates
Detectors

Keywords

  • Acetylation
  • Alditol acetate
  • Empty fruit bunch fiber
  • Methanolysis
  • Monosaccharides

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Kaedah analatikal bagi penentuan monosakarida daripada serabut sawit terawat alkali. / Badri, Khairiah; Juan, Cheah Ai; Hassan, Osman; Wan Mustapha, Wan Aida.

In: Malaysian Journal of Analytical Sciences, Vol. 19, No. 1, 2015, p. 46-54.

Research output: Contribution to journalArticle

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