Analysis of the sodium chloride-dependent respiratory kinetics of wheat mitochondria reveals differential effects on phosphorylating and non-phosphorylating electron transport pathways

R. P. Jacoby, Muhamad Hafiz Che Othman, A. H. Millar, N. L. Taylor

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A number of previous studies have documented the gross response of mitochondrial respiration to salinity treatment, but it is unclear how NaCl directly affects the kinetics of plant phosphorylating and non-phosphorylating electron transport pathways. This study investigates the direct effects of NaCl upon different respiratory pathways in wheat, by measuring rates of isolated mitochondrial oxygen consumption across different substrate oxidation pathways in saline media. We also profile the abundance of respiratory proteins by using targeted selected reaction monitoring (SRM) mass spectrometry of mitochondria isolated from control and salt-treated wheat plants. We show that all pathways of electron transport were inhibited by NaCl concentrations above 400mM; however electron transfer chains showed divergent responses to NaCl concentrations between 0 and 200mM. Stimulation of oxygen consumption was measured in response to NaCl in scenarios where exogenous NADH was provided as substrate and electron flow was coupled to the generation of a proton gradient across the inner membrane. Protein abundance measurements show that several enzymes with activities less affected by NaCl are induced by salinity, whereas enzymes with activities inhibited by NaCl are depleted. These data deepen our understanding of how plant respiration responds to NaCl, offering new mechanistic explanations for the divergent salinity responses of whole-plant respiratory rate in the literature.

Original languageEnglish
Pages (from-to)823-833
Number of pages11
JournalPlant, Cell and Environment
Volume39
Issue number4
DOIs
Publication statusPublished - 1 Apr 2016
Externally publishedYes

Fingerprint

Electron Transport
Sodium Chloride
sodium chloride
electron transfer
Triticum
Salinity
Mitochondria
mitochondria
kinetics
wheat
Oxygen Consumption
Respiration
Electrons
Enzymes
Respiratory Rate
salinity
cell respiration
NAD
oxygen consumption
Protons

Keywords

  • Alternative oxidase
  • Mitochondrial electron transfer
  • Oxidative phosphorylation
  • Respiration
  • SRM mass spectrometry
  • Triticum spp

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

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abstract = "A number of previous studies have documented the gross response of mitochondrial respiration to salinity treatment, but it is unclear how NaCl directly affects the kinetics of plant phosphorylating and non-phosphorylating electron transport pathways. This study investigates the direct effects of NaCl upon different respiratory pathways in wheat, by measuring rates of isolated mitochondrial oxygen consumption across different substrate oxidation pathways in saline media. We also profile the abundance of respiratory proteins by using targeted selected reaction monitoring (SRM) mass spectrometry of mitochondria isolated from control and salt-treated wheat plants. We show that all pathways of electron transport were inhibited by NaCl concentrations above 400mM; however electron transfer chains showed divergent responses to NaCl concentrations between 0 and 200mM. Stimulation of oxygen consumption was measured in response to NaCl in scenarios where exogenous NADH was provided as substrate and electron flow was coupled to the generation of a proton gradient across the inner membrane. Protein abundance measurements show that several enzymes with activities less affected by NaCl are induced by salinity, whereas enzymes with activities inhibited by NaCl are depleted. These data deepen our understanding of how plant respiration responds to NaCl, offering new mechanistic explanations for the divergent salinity responses of whole-plant respiratory rate in the literature.",
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