Analysis of the elemental composition and uptake mechanism of Chlorella sorokiniana for nutrient removal in agricultural wastewater under optimized response surface methodology (RSM) conditions

Research output: Contribution to journalArticle

Abstract

Microalgae exhibit large potential as an alternative to advanced biological nutrient removal in wastewater, but treatment efficiency is greatly influenced by process variables. Therefore, it is necessary to determine the optimum operating conditions for nutrient removal. In this study, Chlorella sorokiniana was used to remove ammonium (NH₄⁺) and phosphate (PO₄³⁻) from palm oil mill effluent. Response surface methodology (RSM) was employed to evaluate the interactions of three main influential factors, i.e., light intensity, photoperiod and inoculum size, and their effects on nutrient removal efficiency. The nutrient preference, biomass elemental composition and nutrient removal mechanism of the microalgae were also investigated. Under the optimum conditions (200 μmol photon m⁻2s⁻1 12 h photoperiod and 28% inoculum size), 93.36% of NH₄⁺ and 94.50% of PO₄³⁻ were successfully removed. The microalgae were found to adjust their internal composition in response to the external concentration. Biomass N and P increased from 6.16 to 8.68% and from 1.0 to 2.21%, respectively. The microalgae preferred NH₄⁺ over organic N, and the removal mechanisms involved not only assimilation for growth but also over-uptake for cellular storage. These findings are highly beneficial for maximizing the nutrient removal potential of microalgae in agricultural wastewater.

LanguageEnglish
Pages673-686
Number of pages14
JournalJournal of Cleaner Production
Volume210
DOIs
Publication statusPublished - 10 Feb 2019

Fingerprint

Biological water treatment
Palm oil
Algae
Wastewater treatment
Microorganisms
Agriculture
Nutrients
Surface properties
Effluents
Biomass
Wastewater
microorganism
alga
effluent
agriculture
wastewater
biomass
Chemical analysis
photoperiod
light intensity

Keywords

  • Biomass nutrient composition
  • C. sorokiniana
  • Nutrient storage
  • Nutrient uptake optimization
  • POME treatment
  • RSM-CCD

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Environmental Science(all)
  • Strategy and Management
  • Industrial and Manufacturing Engineering

Cite this

@article{9a863ad43bb6406b9ef24d06568b813d,
title = "Analysis of the elemental composition and uptake mechanism of Chlorella sorokiniana for nutrient removal in agricultural wastewater under optimized response surface methodology (RSM) conditions",
abstract = "Microalgae exhibit large potential as an alternative to advanced biological nutrient removal in wastewater, but treatment efficiency is greatly influenced by process variables. Therefore, it is necessary to determine the optimum operating conditions for nutrient removal. In this study, Chlorella sorokiniana was used to remove ammonium (NH₄⁺) and phosphate (PO₄³⁻) from palm oil mill effluent. Response surface methodology (RSM) was employed to evaluate the interactions of three main influential factors, i.e., light intensity, photoperiod and inoculum size, and their effects on nutrient removal efficiency. The nutrient preference, biomass elemental composition and nutrient removal mechanism of the microalgae were also investigated. Under the optimum conditions (200 μmol photon m⁻2s⁻1 12 h photoperiod and 28{\%} inoculum size), 93.36{\%} of NH₄⁺ and 94.50{\%} of PO₄³⁻ were successfully removed. The microalgae were found to adjust their internal composition in response to the external concentration. Biomass N and P increased from 6.16 to 8.68{\%} and from 1.0 to 2.21{\%}, respectively. The microalgae preferred NH₄⁺ over organic N, and the removal mechanisms involved not only assimilation for growth but also over-uptake for cellular storage. These findings are highly beneficial for maximizing the nutrient removal potential of microalgae in agricultural wastewater.",
keywords = "Biomass nutrient composition, C. sorokiniana, Nutrient storage, Nutrient uptake optimization, POME treatment, RSM-CCD",
author = "Khalid, {Azianabiha A.Halip} and Zahira Yaakob and {Sheikh Abdullah}, {Siti Rozaimah} and Takriff, {Mohd Sobri}",
year = "2019",
month = "2",
day = "10",
doi = "10.1016/j.jclepro.2018.11.095",
language = "English",
volume = "210",
pages = "673--686",
journal = "Journal of Cleaner Production",
issn = "0959-6526",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Analysis of the elemental composition and uptake mechanism of Chlorella sorokiniana for nutrient removal in agricultural wastewater under optimized response surface methodology (RSM) conditions

AU - Khalid, Azianabiha A.Halip

AU - Yaakob, Zahira

AU - Sheikh Abdullah, Siti Rozaimah

AU - Takriff, Mohd Sobri

PY - 2019/2/10

Y1 - 2019/2/10

N2 - Microalgae exhibit large potential as an alternative to advanced biological nutrient removal in wastewater, but treatment efficiency is greatly influenced by process variables. Therefore, it is necessary to determine the optimum operating conditions for nutrient removal. In this study, Chlorella sorokiniana was used to remove ammonium (NH₄⁺) and phosphate (PO₄³⁻) from palm oil mill effluent. Response surface methodology (RSM) was employed to evaluate the interactions of three main influential factors, i.e., light intensity, photoperiod and inoculum size, and their effects on nutrient removal efficiency. The nutrient preference, biomass elemental composition and nutrient removal mechanism of the microalgae were also investigated. Under the optimum conditions (200 μmol photon m⁻2s⁻1 12 h photoperiod and 28% inoculum size), 93.36% of NH₄⁺ and 94.50% of PO₄³⁻ were successfully removed. The microalgae were found to adjust their internal composition in response to the external concentration. Biomass N and P increased from 6.16 to 8.68% and from 1.0 to 2.21%, respectively. The microalgae preferred NH₄⁺ over organic N, and the removal mechanisms involved not only assimilation for growth but also over-uptake for cellular storage. These findings are highly beneficial for maximizing the nutrient removal potential of microalgae in agricultural wastewater.

AB - Microalgae exhibit large potential as an alternative to advanced biological nutrient removal in wastewater, but treatment efficiency is greatly influenced by process variables. Therefore, it is necessary to determine the optimum operating conditions for nutrient removal. In this study, Chlorella sorokiniana was used to remove ammonium (NH₄⁺) and phosphate (PO₄³⁻) from palm oil mill effluent. Response surface methodology (RSM) was employed to evaluate the interactions of three main influential factors, i.e., light intensity, photoperiod and inoculum size, and their effects on nutrient removal efficiency. The nutrient preference, biomass elemental composition and nutrient removal mechanism of the microalgae were also investigated. Under the optimum conditions (200 μmol photon m⁻2s⁻1 12 h photoperiod and 28% inoculum size), 93.36% of NH₄⁺ and 94.50% of PO₄³⁻ were successfully removed. The microalgae were found to adjust their internal composition in response to the external concentration. Biomass N and P increased from 6.16 to 8.68% and from 1.0 to 2.21%, respectively. The microalgae preferred NH₄⁺ over organic N, and the removal mechanisms involved not only assimilation for growth but also over-uptake for cellular storage. These findings are highly beneficial for maximizing the nutrient removal potential of microalgae in agricultural wastewater.

KW - Biomass nutrient composition

KW - C. sorokiniana

KW - Nutrient storage

KW - Nutrient uptake optimization

KW - POME treatment

KW - RSM-CCD

UR - http://www.scopus.com/inward/record.url?scp=85057205672&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057205672&partnerID=8YFLogxK

U2 - 10.1016/j.jclepro.2018.11.095

DO - 10.1016/j.jclepro.2018.11.095

M3 - Article

VL - 210

SP - 673

EP - 686

JO - Journal of Cleaner Production

T2 - Journal of Cleaner Production

JF - Journal of Cleaner Production

SN - 0959-6526

ER -