Analysis of fucoxanthin content and purification of all-trans-fucoxanthin from turbinaria turbinata and sargassum plagyophyllum by SiO2 open column chromatography and reversed phase-HPLC

Irwandi Jaswir, Dedi Noviendri, Hamzah M. Salleh, Muhammad Taher, Kazuo Miyashita, Nazaruddin Ramli

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Reversed phase-high performance liquid chromatography (RP-HPLC) was used to analyze fucoxanthin content of two species of Malaysian brown seaweeds, Turbinaria turbinate, and Sargassum plagyophyllum. The fucoxanthin contents of T. turbinata and S. plagyophyllum were 0.59 ± 0.08 and 0.71 ± 0.01 mg/g dry-weight, respectively. Ultraviolet spectrum of fucoxanthin showed the maximum absorbance at 450 nm. Furthermore, the HPLC chromatograms of purified all-trans-fucoxanthin from showed one major peak with a retention time of 7.8 min.

    Original languageEnglish
    Pages (from-to)1340-1354
    Number of pages15
    JournalJournal of Liquid Chromatography and Related Technologies
    Volume36
    Issue number10
    DOIs
    Publication statusPublished - 1 Apr 2013

    Fingerprint

    Sargassum
    Column chromatography
    Reverse-Phase Chromatography
    Purification
    High Pressure Liquid Chromatography
    Seaweed
    Turbinates
    High performance liquid chromatography
    Weights and Measures
    fucoxanthin

    Keywords

    • extraction
    • fucoxanthin
    • open column chromatography
    • purification
    • reversed phase-HPLC
    • Sargassum plagyophyllum
    • thin layer chromatography
    • Turbinaria turbinata

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Pharmaceutical Science
    • Biochemistry
    • Clinical Biochemistry

    Cite this

    Analysis of fucoxanthin content and purification of all-trans-fucoxanthin from turbinaria turbinata and sargassum plagyophyllum by SiO2 open column chromatography and reversed phase-HPLC. / Jaswir, Irwandi; Noviendri, Dedi; Salleh, Hamzah M.; Taher, Muhammad; Miyashita, Kazuo; Ramli, Nazaruddin.

    In: Journal of Liquid Chromatography and Related Technologies, Vol. 36, No. 10, 01.04.2013, p. 1340-1354.

    Research output: Contribution to journalArticle

    @article{e5c7b84aabb748878740bb6b6e583f41,
    title = "Analysis of fucoxanthin content and purification of all-trans-fucoxanthin from turbinaria turbinata and sargassum plagyophyllum by SiO2 open column chromatography and reversed phase-HPLC",
    abstract = "Reversed phase-high performance liquid chromatography (RP-HPLC) was used to analyze fucoxanthin content of two species of Malaysian brown seaweeds, Turbinaria turbinate, and Sargassum plagyophyllum. The fucoxanthin contents of T. turbinata and S. plagyophyllum were 0.59 ± 0.08 and 0.71 ± 0.01 mg/g dry-weight, respectively. Ultraviolet spectrum of fucoxanthin showed the maximum absorbance at 450 nm. Furthermore, the HPLC chromatograms of purified all-trans-fucoxanthin from showed one major peak with a retention time of 7.8 min.",
    keywords = "extraction, fucoxanthin, open column chromatography, purification, reversed phase-HPLC, Sargassum plagyophyllum, thin layer chromatography, Turbinaria turbinata",
    author = "Irwandi Jaswir and Dedi Noviendri and Salleh, {Hamzah M.} and Muhammad Taher and Kazuo Miyashita and Nazaruddin Ramli",
    year = "2013",
    month = "4",
    day = "1",
    doi = "10.1080/10826076.2012.691435",
    language = "English",
    volume = "36",
    pages = "1340--1354",
    journal = "Journal of Liquid Chromatography and Related Technologies",
    issn = "1082-6076",
    publisher = "Taylor and Francis Ltd.",
    number = "10",

    }

    TY - JOUR

    T1 - Analysis of fucoxanthin content and purification of all-trans-fucoxanthin from turbinaria turbinata and sargassum plagyophyllum by SiO2 open column chromatography and reversed phase-HPLC

    AU - Jaswir, Irwandi

    AU - Noviendri, Dedi

    AU - Salleh, Hamzah M.

    AU - Taher, Muhammad

    AU - Miyashita, Kazuo

    AU - Ramli, Nazaruddin

    PY - 2013/4/1

    Y1 - 2013/4/1

    N2 - Reversed phase-high performance liquid chromatography (RP-HPLC) was used to analyze fucoxanthin content of two species of Malaysian brown seaweeds, Turbinaria turbinate, and Sargassum plagyophyllum. The fucoxanthin contents of T. turbinata and S. plagyophyllum were 0.59 ± 0.08 and 0.71 ± 0.01 mg/g dry-weight, respectively. Ultraviolet spectrum of fucoxanthin showed the maximum absorbance at 450 nm. Furthermore, the HPLC chromatograms of purified all-trans-fucoxanthin from showed one major peak with a retention time of 7.8 min.

    AB - Reversed phase-high performance liquid chromatography (RP-HPLC) was used to analyze fucoxanthin content of two species of Malaysian brown seaweeds, Turbinaria turbinate, and Sargassum plagyophyllum. The fucoxanthin contents of T. turbinata and S. plagyophyllum were 0.59 ± 0.08 and 0.71 ± 0.01 mg/g dry-weight, respectively. Ultraviolet spectrum of fucoxanthin showed the maximum absorbance at 450 nm. Furthermore, the HPLC chromatograms of purified all-trans-fucoxanthin from showed one major peak with a retention time of 7.8 min.

    KW - extraction

    KW - fucoxanthin

    KW - open column chromatography

    KW - purification

    KW - reversed phase-HPLC

    KW - Sargassum plagyophyllum

    KW - thin layer chromatography

    KW - Turbinaria turbinata

    UR - http://www.scopus.com/inward/record.url?scp=84876118777&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84876118777&partnerID=8YFLogxK

    U2 - 10.1080/10826076.2012.691435

    DO - 10.1080/10826076.2012.691435

    M3 - Article

    AN - SCOPUS:84876118777

    VL - 36

    SP - 1340

    EP - 1354

    JO - Journal of Liquid Chromatography and Related Technologies

    JF - Journal of Liquid Chromatography and Related Technologies

    SN - 1082-6076

    IS - 10

    ER -