Analysis of Biofilms Formation by Cronobacter sp. during Growth in Infant Formula Milk

Aishah Faiqah Mohd Yusof, Pranesha Prabhakaran, Nur Diyana Azli, Norrakiah Abdullah Sani, Wan Syaidatul Aqma Wan Mohd Noor

Research output: Contribution to journalArticle

Abstract

Pacifier nipples are in permanent contact with saliva and with the oral microflora therefore, act as a favoured site for the growth of biofilms. This research was conducted to identify the bacterial biofilms that has been found on the pacifiers that collected from local child nursery and to analyse the formation of biofilms by Cronobacter sp. during growth in infant formula milk. Pacifiers collected were analysed to obtain colony forming unit (CFU) and isolated bacteria were identified using several biochemical tests according to Bergey's Manual. Biofilm assay of three Cronobacter sp. were conducted using 24 wells microtiter plate and stained with 1% of crystal violet solution at different time interval: 6, 12, 18 and 24 h. The hydrophobicity of the bacterial cell suspension was evaluated using bacterial adhesion to hydrocarbons (BATH) method. Extracellular polymeric substances (EPS) analysis was done to identify percentage of carbohydrate and protein content by using phenol sulphuric acid method and Bradford method, respectively. The results obtained showed that the normal microflora bacteria were the most abundant microorganisms that were found on the pacifier with the main genus isolated was Staphylococcus sp., Enterobacteriaceae sp. and Clostridium sp. Based on biofilm and EPS analysis, Cronobacter sakazakii formed a strong biofilms after 18 h, with carbohydrate was identified as main component of EPS.

Original languageEnglish
Pages (from-to)903-908
Number of pages6
JournalSains Malaysiana
Volume46
Issue number6
DOIs
Publication statusPublished - 1 Jun 2017

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milk
biofilm
carbohydrate
saliva
bacterium
hydrophobicity
adhesion
sulfuric acid
analysis
infant
phenol
microorganism
assay
crystal
hydrocarbon
well
protein
method

Keywords

  • Cell surface hydrophobicity
  • Extracellular polymeric substances (EPS)
  • Staphylococcus sp

ASJC Scopus subject areas

  • General

Cite this

Analysis of Biofilms Formation by Cronobacter sp. during Growth in Infant Formula Milk. / Yusof, Aishah Faiqah Mohd; Prabhakaran, Pranesha; Azli, Nur Diyana; Abdullah Sani, Norrakiah; Wan Mohd Noor, Wan Syaidatul Aqma.

In: Sains Malaysiana, Vol. 46, No. 6, 01.06.2017, p. 903-908.

Research output: Contribution to journalArticle

Yusof, Aishah Faiqah Mohd ; Prabhakaran, Pranesha ; Azli, Nur Diyana ; Abdullah Sani, Norrakiah ; Wan Mohd Noor, Wan Syaidatul Aqma. / Analysis of Biofilms Formation by Cronobacter sp. during Growth in Infant Formula Milk. In: Sains Malaysiana. 2017 ; Vol. 46, No. 6. pp. 903-908.
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