Analysis of Antarctic proteobacteria by PCR fingerprinting and screening for antimicrobial secondary metabolites.

L. H. Lee, Y. K. Cheah, Nurul Syakima Ab Mutalib, M. S. Shiran, Y. L. Tang, H. P. Lin, K. Hong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Fifty-seven proteobacterium species were successfully isolated from soils of Barrientos Island of the Antarctic using 11 different isolation media. Analysis of 16S rDNA sequencing of these isolates showed that they belonged to eight different genera, namely Bradyrhizobium, Sphingomonas, Methylobacterium, Caulobacter, Paracoccus, Ralstonia, Rhizobium, and Staphylococcus. All isolates were studied for capability of producing antimicrobial and antifungal secondary metabolites using high-throughput screening models. Approximately 23 (13/57) and 2% (1/57) of isolates inhibited growth of Candida albicans ATCC 10231(T) and Staphylococcus aureus ATCC 51650(T), respectively. These results indicated that proteobacterium species isolates from Antarctic could serve as potential source of useful bioactive metabolites. Enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting produced nine clusters and 13 single isolates, with a high D value of 0.9248. RAPD fingerprinting produced six clusters and 13 single isolates, with a relatively low D value of 0.7776. ERIC-PCR analysis proved to have better discrimination capability than RAPD analysis and generated better clustering for all proteobacterium species isolates. We conclude that ERIC-PCR is a robust, reliable and rapid molecular typing method for discriminating different genera of proteobacteria.

Original languageEnglish
Pages (from-to)1627-1641
Number of pages15
JournalGenetics and molecular research : GMR
Volume11
Issue number2
DOIs
Publication statusPublished - 2012

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Proteobacteria
Polymerase Chain Reaction
Paracoccus
Caulobacter
Ralstonia
Methylobacterium
Sphingomonas
Bradyrhizobium
Molecular Typing
Rhizobium
Ribosomal DNA
Candida albicans
Staphylococcus
Islands
Cluster Analysis
Staphylococcus aureus
Soil
Growth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Analysis of Antarctic proteobacteria by PCR fingerprinting and screening for antimicrobial secondary metabolites. / Lee, L. H.; Cheah, Y. K.; Ab Mutalib, Nurul Syakima; Shiran, M. S.; Tang, Y. L.; Lin, H. P.; Hong, K.

In: Genetics and molecular research : GMR, Vol. 11, No. 2, 2012, p. 1627-1641.

Research output: Contribution to journalArticle

Lee, L. H. ; Cheah, Y. K. ; Ab Mutalib, Nurul Syakima ; Shiran, M. S. ; Tang, Y. L. ; Lin, H. P. ; Hong, K. / Analysis of Antarctic proteobacteria by PCR fingerprinting and screening for antimicrobial secondary metabolites. In: Genetics and molecular research : GMR. 2012 ; Vol. 11, No. 2. pp. 1627-1641.
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