Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell

Shahrul Hisham Zainal Ariffin, Nur Akmal Mohamed Rozali, Rohaya Megat Abdul Wahab, Sahidan Senafi, Intan Zarina Zainol Abidin, Zaidah Zainal Ariffin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Transplantation of stem cells requires a huge amount of cells, deeming the expansion of the cells in vitro necessary. The aim of this study is to define the optimal combination of basal medium and serum for the expansion of suspension peripheral blood mononucleated stem cells (PBMNSCs) without resulting in loss in the differentiation potential. Mononucleated cells were isolated from both mice and human peripheral blood samples through gradient centrifugation and expanded in α-MEM, RPMI, MEM or DMEM supplemented with either NBCS or FBS. The suspension cells were then differentiated to osteoblast. Our data suggested that α-MEM supplemented with 10 % (v/v) NBCS gives the highest fold increase after 14 days of culture for both mice and human PBMNSCs, which were ~1.51 and ~2.01 times, respectively. The suspension PBMNSCs in the respective medium were also able to maintain osteoblast differentiation potential as supported by the significant increase in ALP specific activity. The cells are also viable during the differentiated states when using this media. All these data strongly suggested that α-MEM supplemented with 10 % NBCS is the best media for the expansion of both mouse and human suspension PBMNSCs.

Original languageEnglish
JournalCytotechnology
DOIs
Publication statusAccepted/In press - 1 Aug 2015

Fingerprint

Stem cells
Suspensions
Blood
Serum
Osteoblasts
Centrifugation
Stem Cell Transplantation
Cell culture
Peripheral Blood Stem Cells
In Vitro Techniques

Keywords

  • Basal media
  • Cell differentiation
  • Cell expansion
  • Peripheral blood
  • Serum
  • Suspension stem cell

ASJC Scopus subject areas

  • Cell Biology
  • Biotechnology
  • Biomedical Engineering
  • Bioengineering
  • Clinical Biochemistry

Cite this

Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell. / Zainal Ariffin, Shahrul Hisham; Mohamed Rozali, Nur Akmal; Megat Abdul Wahab, Rohaya; Senafi, Sahidan; Zainol Abidin, Intan Zarina; Zainal Ariffin, Zaidah.

In: Cytotechnology, 01.08.2015.

Research output: Contribution to journalArticle

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AU - Senafi, Sahidan

AU - Zainol Abidin, Intan Zarina

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