An optical sensor based on immobilized copper(II) ions for the determination of free glutamate in food samples

Noor Zuhartini Md Muslim, Musa Ahmad, Yook Heng Lee, Bahruddin Saad

Research output: Contribution to journalArticle

Abstract

An optical fiber chemical sensor for the determination of free glutamate in food samples was fabricated based on the immobilization of 0.1 M copper(II) nitrate trihydrate onto sol-gel glass powder which was then mixed with methyl cellulose to form a pellet. A distinctive colour change from light blue to dark blue was observed in the presence of glutamate in less than 1 min. The colour change was measured by reflectance spectrophotometer at 691 nm. A linear relationship between the reflectance intensity and glutamate concentration was observed in the range of 12.5 to 500 mM with a limit of detection of 10.6 mM. This method is also reproducible with a relative standard deviation of less than 5%, no effect on pH of the glutamate solution and a good recovery of above 80%. The sensor was used for the determination of glutamate in common food items such as soups and flavor enhancers. The results obtained from the fabricated sensor were found to be comparable with HPLC method.

Original languageEnglish
Pages (from-to)707-713
Number of pages7
JournalSains Malaysiana
Volume47
Issue number4
DOIs
Publication statusPublished - 1 Apr 2018

Fingerprint

Optical sensors
Copper
Glutamic Acid
Ions
Fiber optic chemical sensors
Color
Methylcellulose
Flavors
Spectrophotometers
Sensors
Nitrates
Powders
Sol-gels
Recovery
Glass

Keywords

  • Copper(II) nitrate trihydrate
  • Free glutamate
  • Optical fiber chemical sensor
  • Sol-gel glass powder

ASJC Scopus subject areas

  • General

Cite this

An optical sensor based on immobilized copper(II) ions for the determination of free glutamate in food samples. / Md Muslim, Noor Zuhartini; Ahmad, Musa; Lee, Yook Heng; Saad, Bahruddin.

In: Sains Malaysiana, Vol. 47, No. 4, 01.04.2018, p. 707-713.

Research output: Contribution to journalArticle

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