An optical biosensor from green fluorescent Escherichia coli for the evaluation of single and combined heavy metal toxicities

Dedi Futra, Yook Heng Lee, Asmat Ahmad, Salmijah Surif, Ling Ling Tan @ Chong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A fluorescence-based fiber optic toxicity biosensor based on genetically modified Escherichia coli (E. coli) with green fluorescent protein (GFP) was developed for the evaluation of the toxicity of several hazardous heavy metal ions. The toxic metals include Cu(II), Cd(II), Pb(II), Zn(II), Cr(VI), Co(II), Ni(II), Ag(I) and Fe(III). The optimum fluorescence excitation and emission wavelengths of the optical biosensor were 400 ± 2 nm and 485 ± 2 nm, respectively. Based on the toxicity observed under optimal conditions, the detection limits of Cu(II), Cd(II), Pb(II), Zn(II), Cr(VI), Co(II), Ni(II), Ag(I) and Fe(III) that can be detected using the toxicity biosensor were at 0.04, 0.32, 0.46, 2.80, 100, 250, 400, 720 and 2600 μg/L, respectively. The repeatability and reproducibility of the proposed biosensor were 3.5%–4.8% RSD (relative standard deviation) and 3.6%–5.1% RSD (n = 8), respectively. The biosensor response was stable for at least five weeks, and demonstrated higher sensitivity towards metal toxicity evaluation when compared to a conventional Microtox assay.

Original languageEnglish
Pages (from-to)12668-12681
Number of pages14
JournalSensors (Switzerland)
Volume15
Issue number6
DOIs
Publication statusPublished - 28 May 2015

Fingerprint

Biosensing Techniques
heavy metals
Escherichia
Heavy Metals
bioinstrumentation
Biosensors
toxicity
Escherichia coli
Heavy metals
Toxicity
evaluation
standard deviation
Fluorescence
Metals
Heavy Ions
fluorescence
Poisons
Green Fluorescent Proteins
Heavy ions
metals

Keywords

  • GFP E. Coli
  • Hazardous heavy metal
  • Optical fiber biosensor
  • Whole cell biosensor

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Analytical Chemistry
  • Biochemistry

Cite this

An optical biosensor from green fluorescent Escherichia coli for the evaluation of single and combined heavy metal toxicities. / Futra, Dedi; Lee, Yook Heng; Ahmad, Asmat; Surif, Salmijah; Tan @ Chong, Ling Ling.

In: Sensors (Switzerland), Vol. 15, No. 6, 28.05.2015, p. 12668-12681.

Research output: Contribution to journalArticle

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