An optical biosensor based on immobilization of lacease and MBTH in stacked films for the detection of catechol

Jaafar Abdullah, Musa Ahmad, Yook Heng Lee, Nadarajah Karuppiah, Hamidah Sidek

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The fabrication of an optical biosensor by using stacked films where 3-methyl2-benzothiazolinone hydrazone (MBTH) was immobilized in a hybrid nafion/sol-gel silicate film and lacease in a chitosan film for the detection of phenolic compounds was described. Quinone and/or phenoxy radical product from the enzymatic oxidation of phenolic compounds was allowed to couple with MBTH to form a colored azo-dye product for spectrophometric detection. The biosensor demonstrated a linear response to catechol concentration range of 0.5-8.0 mM with detection limit of 0.33 mM and response time of 10 min. The reproducibility of the fabricated biosensor was good with RSD value of 5.3 % (n = 8) and stable for at least 2 months. The use of the hybrid materials of nafion/sol-gel silicate to immobilize lacease has altered the selectivity of the enzyme to various phenolic compounds such as catechol, guaicol, o-cresol and m-cresol when compared to the nonimmobilized enzyme. When immobilized in this hybrid film, the biosensor response only to catechol and not other phenolic compounds investigated. Immobilization in this hybrid material has enable the biosensor to be more selective to catechol compared with the nonimmobilized enzyme. This shows that by a careful selection of different immobilization matrices, the selectivity of an enzyme can be modified to yield a biosensor with good selectivity towards certain targeted analytes.

Original languageEnglish
Pages (from-to)2238-2250
Number of pages13
JournalSensors
Volume7
Issue number10
Publication statusPublished - Oct 2007

Fingerprint

Hydrazones
hydrazones
Biosensing Techniques
immobilization
bioinstrumentation
Biosensors
Immobilization
enzymes
Enzymes
Silicates
cresols
Catalyst selectivity
selectivity
Hybrid materials
Polymethyl Methacrylate
Sol-gels
silicates
Azo Compounds
Gels
gels

Keywords

  • Biosensor
  • Lacease
  • MBTH
  • Phenolic compounds

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

An optical biosensor based on immobilization of lacease and MBTH in stacked films for the detection of catechol. / Abdullah, Jaafar; Ahmad, Musa; Lee, Yook Heng; Karuppiah, Nadarajah; Sidek, Hamidah.

In: Sensors, Vol. 7, No. 10, 10.2007, p. 2238-2250.

Research output: Contribution to journalArticle

Abdullah, J, Ahmad, M, Lee, YH, Karuppiah, N & Sidek, H 2007, 'An optical biosensor based on immobilization of lacease and MBTH in stacked films for the detection of catechol', Sensors, vol. 7, no. 10, pp. 2238-2250.
Abdullah, Jaafar ; Ahmad, Musa ; Lee, Yook Heng ; Karuppiah, Nadarajah ; Sidek, Hamidah. / An optical biosensor based on immobilization of lacease and MBTH in stacked films for the detection of catechol. In: Sensors. 2007 ; Vol. 7, No. 10. pp. 2238-2250.
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