An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples

Nicolle Breusing, Tilman Grune, Luka Andrisic, Mustafa Atalay, Grzegorz Bartosz, Fiorella Biasi, Suzana Borovic, Laura Bravo, Isidre Casals, Rosario Casillas, Anca Dinischiotu, Joanna Drzewinska, Heidemarie Faber, Norsyahida Mohd Fauzi, Agnieszka Gajewska, Juan Gambini, Daniela Gradinaru, Tarja Kokkola, Antonin Lojek, Wojciech ŁuczajDenisa Margina, Cinzia Mascia, Raquel Mateos, Andreas Meinitzer, Mara Teresa Mitjavila, Lidija Mrakovcic, Maria Cristina Munteanu, Martina Podborska, Giuseppe Poli, Paulina Sicinska, Elzbieta Skrzydlewska, Jose Vina, Ingrid Wiswedel, Neven Zarkovic, Sieglinde Zelzer, Corinne M. Spickett

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F2-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.

Original languageEnglish
Pages (from-to)1203-1215
Number of pages13
JournalFree Radical Research
Volume44
Issue number10
DOIs
Publication statusPublished - Oct 2010
Externally publishedYes

Fingerprint

Plasma (human)
Lipid Peroxidation
Malondialdehyde
Lipids
F2-Isoprostanes
High Pressure Liquid Chromatography
Isoprostanes
Plasmas
Oxidative stress
Validation Studies
Edetic Acid
Dosimetry
Oxidative Stress
Irradiation

Keywords

  • 4-hydroxynonenal
  • F2-isoprostanes
  • malondialdehyde
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry

Cite this

Breusing, N., Grune, T., Andrisic, L., Atalay, M., Bartosz, G., Biasi, F., ... Spickett, C. M. (2010). An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples. Free Radical Research, 44(10), 1203-1215. https://doi.org/10.3109/10715762.2010.499907

An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples. / Breusing, Nicolle; Grune, Tilman; Andrisic, Luka; Atalay, Mustafa; Bartosz, Grzegorz; Biasi, Fiorella; Borovic, Suzana; Bravo, Laura; Casals, Isidre; Casillas, Rosario; Dinischiotu, Anca; Drzewinska, Joanna; Faber, Heidemarie; Mohd Fauzi, Norsyahida; Gajewska, Agnieszka; Gambini, Juan; Gradinaru, Daniela; Kokkola, Tarja; Lojek, Antonin; Łuczaj, Wojciech; Margina, Denisa; Mascia, Cinzia; Mateos, Raquel; Meinitzer, Andreas; Mitjavila, Mara Teresa; Mrakovcic, Lidija; Munteanu, Maria Cristina; Podborska, Martina; Poli, Giuseppe; Sicinska, Paulina; Skrzydlewska, Elzbieta; Vina, Jose; Wiswedel, Ingrid; Zarkovic, Neven; Zelzer, Sieglinde; Spickett, Corinne M.

In: Free Radical Research, Vol. 44, No. 10, 10.2010, p. 1203-1215.

Research output: Contribution to journalArticle

Breusing, N, Grune, T, Andrisic, L, Atalay, M, Bartosz, G, Biasi, F, Borovic, S, Bravo, L, Casals, I, Casillas, R, Dinischiotu, A, Drzewinska, J, Faber, H, Mohd Fauzi, N, Gajewska, A, Gambini, J, Gradinaru, D, Kokkola, T, Lojek, A, Łuczaj, W, Margina, D, Mascia, C, Mateos, R, Meinitzer, A, Mitjavila, MT, Mrakovcic, L, Munteanu, MC, Podborska, M, Poli, G, Sicinska, P, Skrzydlewska, E, Vina, J, Wiswedel, I, Zarkovic, N, Zelzer, S & Spickett, CM 2010, 'An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples', Free Radical Research, vol. 44, no. 10, pp. 1203-1215. https://doi.org/10.3109/10715762.2010.499907
Breusing, Nicolle ; Grune, Tilman ; Andrisic, Luka ; Atalay, Mustafa ; Bartosz, Grzegorz ; Biasi, Fiorella ; Borovic, Suzana ; Bravo, Laura ; Casals, Isidre ; Casillas, Rosario ; Dinischiotu, Anca ; Drzewinska, Joanna ; Faber, Heidemarie ; Mohd Fauzi, Norsyahida ; Gajewska, Agnieszka ; Gambini, Juan ; Gradinaru, Daniela ; Kokkola, Tarja ; Lojek, Antonin ; Łuczaj, Wojciech ; Margina, Denisa ; Mascia, Cinzia ; Mateos, Raquel ; Meinitzer, Andreas ; Mitjavila, Mara Teresa ; Mrakovcic, Lidija ; Munteanu, Maria Cristina ; Podborska, Martina ; Poli, Giuseppe ; Sicinska, Paulina ; Skrzydlewska, Elzbieta ; Vina, Jose ; Wiswedel, Ingrid ; Zarkovic, Neven ; Zelzer, Sieglinde ; Spickett, Corinne M. / An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples. In: Free Radical Research. 2010 ; Vol. 44, No. 10. pp. 1203-1215.
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AU - Atalay, Mustafa

AU - Bartosz, Grzegorz

AU - Biasi, Fiorella

AU - Borovic, Suzana

AU - Bravo, Laura

AU - Casals, Isidre

AU - Casillas, Rosario

AU - Dinischiotu, Anca

AU - Drzewinska, Joanna

AU - Faber, Heidemarie

AU - Mohd Fauzi, Norsyahida

AU - Gajewska, Agnieszka

AU - Gambini, Juan

AU - Gradinaru, Daniela

AU - Kokkola, Tarja

AU - Lojek, Antonin

AU - Łuczaj, Wojciech

AU - Margina, Denisa

AU - Mascia, Cinzia

AU - Mateos, Raquel

AU - Meinitzer, Andreas

AU - Mitjavila, Mara Teresa

AU - Mrakovcic, Lidija

AU - Munteanu, Maria Cristina

AU - Podborska, Martina

AU - Poli, Giuseppe

AU - Sicinska, Paulina

AU - Skrzydlewska, Elzbieta

AU - Vina, Jose

AU - Wiswedel, Ingrid

AU - Zarkovic, Neven

AU - Zelzer, Sieglinde

AU - Spickett, Corinne M.

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