An in-house assay for serum deoxythymidine kinase.

L. H. Seah, S. H. Ton, S. K. Cheong, Noor Hamidah Hussin

Research output: Contribution to journalArticle

Abstract

An in-house method which utilizes 14C-thymidine as a substrate was used to assay deoxythymidine kinase in serum. The method is sensitive enough to detect normal levels of serum deoxythymidine kinase and the assay procedure also enables rapid handling of multiple samples. With a total reaction volume of 60 ul, the enzyme reaction was found to be linear with concentrations for up to 650 U/L of TK activity. On studying serum deoxythymidine kinase (s-TK) activity with incubation time, there was a proportional increase in activity with the length of incubation time. "Within-batch" precision showed a coefficient of variation (CV) of 4.7% for serum with extremely high s-TK levels and a CV of 8.8% for serum with normal s-TK levels. S-TK showed a CV of less than 16.0% in its activity when stored at -8 degrees C and at -20 degrees C. The normal reference range obtained for s-TK activity was 8.6 +/- 7.5 U/L.

Original languageEnglish
Pages (from-to)109-113
Number of pages5
JournalThe Malaysian journal of pathology
Volume13
Issue number2
Publication statusPublished - Dec 1991

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Thymidine Kinase
Serum
Reference Values
Thymidine
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Seah, L. H., Ton, S. H., Cheong, S. K., & Hussin, N. H. (1991). An in-house assay for serum deoxythymidine kinase. The Malaysian journal of pathology, 13(2), 109-113.

An in-house assay for serum deoxythymidine kinase. / Seah, L. H.; Ton, S. H.; Cheong, S. K.; Hussin, Noor Hamidah.

In: The Malaysian journal of pathology, Vol. 13, No. 2, 12.1991, p. 109-113.

Research output: Contribution to journalArticle

Seah, LH, Ton, SH, Cheong, SK & Hussin, NH 1991, 'An in-house assay for serum deoxythymidine kinase.', The Malaysian journal of pathology, vol. 13, no. 2, pp. 109-113.
Seah LH, Ton SH, Cheong SK, Hussin NH. An in-house assay for serum deoxythymidine kinase. The Malaysian journal of pathology. 1991 Dec;13(2):109-113.
Seah, L. H. ; Ton, S. H. ; Cheong, S. K. ; Hussin, Noor Hamidah. / An in-house assay for serum deoxythymidine kinase. In: The Malaysian journal of pathology. 1991 ; Vol. 13, No. 2. pp. 109-113.
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