An immunohistochemical study of retinoblastoma gene product in normal, premalignant and malignant tissues of the uterine cervix

M. D. Noraini, Madya Ali Siti-Aishah, S. W. Kwan

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The retinoblastoma gene was the first tumour suppressor gene identified that was altered not only in retinoblastomas but has been described in a wide variety of human neoplasms. The retinoblastoma gene encodes a nuclear phosphoprotein that in its hypophosphorylated state plays an important role in regulating the cell cycle, thus preventing from tumour formation. Expression of retinoblastoma gene protein product (pRB) was investigated in 118 formalin-fixed, paraffin-embedded cervical tissues by immunohistochemistry using commercially available antibody directed against RB protein. Ten normal ectocervical epithelium, 16 cervical intraepithelial neoplasia (CIN) I, 13 CIN II, 14 CIN III, 53 invasive squamous cell carcinoma, 11 adenocarcinoma and 1 small cell carcinoma were selected for this study. The proportions of pRB-positive cells as well as the extent of pRB expression in ectocervical squamous epithelium were assessed and compared among the lesions. The pRB expression was observed in 100% of normal ectocervical epithelium (n=10), 100 % of CIN lesions (n=43) and 98.5 % of invasive carcinoma of the uterine cervix (n=65) and were statistically significant when CIN or CIN/invasive were compared to normal cases (P < 0.01, P < 0.05 respectively). While in invasive squamous cell carcinoma (SCC, 81.8% (9/11) pRB-positive cells were found in much higher percentages in well differentiated SCC compared to 64.3% (18/28) of moderately differentiated cases and only 7.1% (1/14) of poorly differentiated SCC (P < 0.01, respectively). The results of this study suggest that loss of RB protein expression is rare in carcinoma of the uterine cervix and this protein may be important in the pathogenesis of cervical carcinoma.

    Original languageEnglish
    Pages (from-to)52-59
    Number of pages8
    JournalMalaysian Journal of Medical Sciences
    Volume10
    Issue number1
    Publication statusPublished - Jan 2003

    Fingerprint

    Retinoblastoma Genes
    Cervical Intraepithelial Neoplasia
    Cervix Uteri
    Epithelium
    Carcinoma
    Squamous Cell Carcinoma
    Proteins
    Retinoblastoma Protein
    Small Cell Carcinoma
    Retinoblastoma
    Phosphoproteins
    Tumor Suppressor Genes
    Paraffin
    Formaldehyde
    Neoplasms
    Cell Cycle
    Adenocarcinoma
    Immunohistochemistry
    Antibodies

    Keywords

    • Immunohistochemistry
    • pRB
    • Uterine cervix

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

    An immunohistochemical study of retinoblastoma gene product in normal, premalignant and malignant tissues of the uterine cervix. / Noraini, M. D.; Siti-Aishah, Madya Ali; Kwan, S. W.

    In: Malaysian Journal of Medical Sciences, Vol. 10, No. 1, 01.2003, p. 52-59.

    Research output: Contribution to journalArticle

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    abstract = "The retinoblastoma gene was the first tumour suppressor gene identified that was altered not only in retinoblastomas but has been described in a wide variety of human neoplasms. The retinoblastoma gene encodes a nuclear phosphoprotein that in its hypophosphorylated state plays an important role in regulating the cell cycle, thus preventing from tumour formation. Expression of retinoblastoma gene protein product (pRB) was investigated in 118 formalin-fixed, paraffin-embedded cervical tissues by immunohistochemistry using commercially available antibody directed against RB protein. Ten normal ectocervical epithelium, 16 cervical intraepithelial neoplasia (CIN) I, 13 CIN II, 14 CIN III, 53 invasive squamous cell carcinoma, 11 adenocarcinoma and 1 small cell carcinoma were selected for this study. The proportions of pRB-positive cells as well as the extent of pRB expression in ectocervical squamous epithelium were assessed and compared among the lesions. The pRB expression was observed in 100{\%} of normal ectocervical epithelium (n=10), 100 {\%} of CIN lesions (n=43) and 98.5 {\%} of invasive carcinoma of the uterine cervix (n=65) and were statistically significant when CIN or CIN/invasive were compared to normal cases (P < 0.01, P < 0.05 respectively). While in invasive squamous cell carcinoma (SCC, 81.8{\%} (9/11) pRB-positive cells were found in much higher percentages in well differentiated SCC compared to 64.3{\%} (18/28) of moderately differentiated cases and only 7.1{\%} (1/14) of poorly differentiated SCC (P < 0.01, respectively). The results of this study suggest that loss of RB protein expression is rare in carcinoma of the uterine cervix and this protein may be important in the pathogenesis of cervical carcinoma.",
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