An evaluation of accelerated Portland cement as a restorative material

Dalia Abdullah, T. R. Pitt Ford, S. Papaioannou, J. Nicholson, F. McDonald

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Biocompatibility of two variants of accelerated Portland cement (APC) were investigated in vitro by observing the cytomorphology of SaOS-2 osteosarcoma cells in the presence of test materials and the effect of these materials on the expression of markers of bone remodelling. Glass ionomer cement (GIC), mineral trioxide aggregate (MTA) and unmodified Portland cement (RC) were used for comparison. A direct contact assay was undertaken in four samples of each test material, collected at 12, 24, 48 and 72h. Cell morphology was observed using scanning electron microscopy (SEM) and scored. Culture media were collected for cytokine quantification using enzyme-linked immunosorbent assay (ELISA). On SEM evaluation, healthy SaOS-2 cells were found adhering onto the surfaces of APC variants, RC and MTA. In contrast, rounded and dying cells were observed on GIC. Using ELISA, levels of interleukin (IL)-1β, IL-6, IL-18 and OC were significantly higher in APC variants compared with controls and GIC (p<0.01), but these levels of cytokines were not statistically significant compared with MTA. The results of this study provide evidence that both APC variants are non-toxic and may have potential to promote bone healing. Further development of APC is indicated to produce a viable dental restorative material and possibly a material for orthopaedic application.

Original languageEnglish
Pages (from-to)4001-4010
Number of pages10
JournalBiomaterials
Volume23
Issue number19
DOIs
Publication statusPublished - 2002

Fingerprint

Portland cement
Glass Ionomer Cements
Ionomers
Assays
Immunosorbents
Minerals
Electron Scanning Microscopy
Bone
Glass
Enzyme-Linked Immunosorbent Assay
Dental materials
Cytokines
Enzymes
Dental Materials
Scanning electron microscopy
Interleukin-18
Bone Remodeling
Orthopedics
Osteosarcoma
Bone cement

Keywords

  • Cytokines
  • Dental material
  • Portland cement

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Abdullah, D., Pitt Ford, T. R., Papaioannou, S., Nicholson, J., & McDonald, F. (2002). An evaluation of accelerated Portland cement as a restorative material. Biomaterials, 23(19), 4001-4010. https://doi.org/10.1016/S0142-9612(02)00147-3

An evaluation of accelerated Portland cement as a restorative material. / Abdullah, Dalia; Pitt Ford, T. R.; Papaioannou, S.; Nicholson, J.; McDonald, F.

In: Biomaterials, Vol. 23, No. 19, 2002, p. 4001-4010.

Research output: Contribution to journalArticle

Abdullah, D, Pitt Ford, TR, Papaioannou, S, Nicholson, J & McDonald, F 2002, 'An evaluation of accelerated Portland cement as a restorative material', Biomaterials, vol. 23, no. 19, pp. 4001-4010. https://doi.org/10.1016/S0142-9612(02)00147-3
Abdullah, Dalia ; Pitt Ford, T. R. ; Papaioannou, S. ; Nicholson, J. ; McDonald, F. / An evaluation of accelerated Portland cement as a restorative material. In: Biomaterials. 2002 ; Vol. 23, No. 19. pp. 4001-4010.
@article{cf709cfa3ece4098b98fd744f8f0b762,
title = "An evaluation of accelerated Portland cement as a restorative material",
abstract = "Biocompatibility of two variants of accelerated Portland cement (APC) were investigated in vitro by observing the cytomorphology of SaOS-2 osteosarcoma cells in the presence of test materials and the effect of these materials on the expression of markers of bone remodelling. Glass ionomer cement (GIC), mineral trioxide aggregate (MTA) and unmodified Portland cement (RC) were used for comparison. A direct contact assay was undertaken in four samples of each test material, collected at 12, 24, 48 and 72h. Cell morphology was observed using scanning electron microscopy (SEM) and scored. Culture media were collected for cytokine quantification using enzyme-linked immunosorbent assay (ELISA). On SEM evaluation, healthy SaOS-2 cells were found adhering onto the surfaces of APC variants, RC and MTA. In contrast, rounded and dying cells were observed on GIC. Using ELISA, levels of interleukin (IL)-1β, IL-6, IL-18 and OC were significantly higher in APC variants compared with controls and GIC (p<0.01), but these levels of cytokines were not statistically significant compared with MTA. The results of this study provide evidence that both APC variants are non-toxic and may have potential to promote bone healing. Further development of APC is indicated to produce a viable dental restorative material and possibly a material for orthopaedic application.",
keywords = "Cytokines, Dental material, Portland cement",
author = "Dalia Abdullah and {Pitt Ford}, {T. R.} and S. Papaioannou and J. Nicholson and F. McDonald",
year = "2002",
doi = "10.1016/S0142-9612(02)00147-3",
language = "English",
volume = "23",
pages = "4001--4010",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "19",

}

TY - JOUR

T1 - An evaluation of accelerated Portland cement as a restorative material

AU - Abdullah, Dalia

AU - Pitt Ford, T. R.

AU - Papaioannou, S.

AU - Nicholson, J.

AU - McDonald, F.

PY - 2002

Y1 - 2002

N2 - Biocompatibility of two variants of accelerated Portland cement (APC) were investigated in vitro by observing the cytomorphology of SaOS-2 osteosarcoma cells in the presence of test materials and the effect of these materials on the expression of markers of bone remodelling. Glass ionomer cement (GIC), mineral trioxide aggregate (MTA) and unmodified Portland cement (RC) were used for comparison. A direct contact assay was undertaken in four samples of each test material, collected at 12, 24, 48 and 72h. Cell morphology was observed using scanning electron microscopy (SEM) and scored. Culture media were collected for cytokine quantification using enzyme-linked immunosorbent assay (ELISA). On SEM evaluation, healthy SaOS-2 cells were found adhering onto the surfaces of APC variants, RC and MTA. In contrast, rounded and dying cells were observed on GIC. Using ELISA, levels of interleukin (IL)-1β, IL-6, IL-18 and OC were significantly higher in APC variants compared with controls and GIC (p<0.01), but these levels of cytokines were not statistically significant compared with MTA. The results of this study provide evidence that both APC variants are non-toxic and may have potential to promote bone healing. Further development of APC is indicated to produce a viable dental restorative material and possibly a material for orthopaedic application.

AB - Biocompatibility of two variants of accelerated Portland cement (APC) were investigated in vitro by observing the cytomorphology of SaOS-2 osteosarcoma cells in the presence of test materials and the effect of these materials on the expression of markers of bone remodelling. Glass ionomer cement (GIC), mineral trioxide aggregate (MTA) and unmodified Portland cement (RC) were used for comparison. A direct contact assay was undertaken in four samples of each test material, collected at 12, 24, 48 and 72h. Cell morphology was observed using scanning electron microscopy (SEM) and scored. Culture media were collected for cytokine quantification using enzyme-linked immunosorbent assay (ELISA). On SEM evaluation, healthy SaOS-2 cells were found adhering onto the surfaces of APC variants, RC and MTA. In contrast, rounded and dying cells were observed on GIC. Using ELISA, levels of interleukin (IL)-1β, IL-6, IL-18 and OC were significantly higher in APC variants compared with controls and GIC (p<0.01), but these levels of cytokines were not statistically significant compared with MTA. The results of this study provide evidence that both APC variants are non-toxic and may have potential to promote bone healing. Further development of APC is indicated to produce a viable dental restorative material and possibly a material for orthopaedic application.

KW - Cytokines

KW - Dental material

KW - Portland cement

UR - http://www.scopus.com/inward/record.url?scp=0036780134&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036780134&partnerID=8YFLogxK

U2 - 10.1016/S0142-9612(02)00147-3

DO - 10.1016/S0142-9612(02)00147-3

M3 - Article

C2 - 12162333

AN - SCOPUS:0036780134

VL - 23

SP - 4001

EP - 4010

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 19

ER -