An ELISA-disc procedure for antibodies to Pseudomonas pseudomallei: application for a serological study of melioidosis in an endemic area

Mohammed Noor Embi, D. Devarajoo, R. Mohamed, G. Ismail

Research output: Contribution to journalArticle

Abstract

The optimization and development of an ELISA-disc procedure for the detection of antibodies to whole cell surface antigens and purified exotoxin of Pseudomonas pseudomallei is described. Comparison of the serum agglutination test (SAT), the serum based enzyme-linked immunosorbent assay (ELISA) and the ELISA-disc procedures used on goat and human sera demonstrated a high correlation in their ability to detect antibodies specific for P. pseudomallei antigens. A serological survey using the ELISA-disc method was carried out on a normal human population in Sabah, Malaysia, an area known to be endemic for melioidosis. The prevalances of antibodies towards cell surface antigens and exotoxin of P. pseudomallei were 28% and 8%, respectively. As a procedure, the ELISA-disc technique reported here is technically simple and provides savings in costs and is thus deemed suitable for seroepidemiological surveillance of melioidosis in remote areas of South-East Asia.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalWorld Journal of Microbiology & Biotechnology
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 1993

Fingerprint

Burkholderia pseudomallei
Melioidosis
Immunosorbents
serological surveys
Assays
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
antibodies
Antibodies
exotoxins
Enzymes
Exotoxins
Malaysia
surface antigens
Surface Antigens
Serum
Agglutination Tests
Far East
Cost Savings
agglutination tests

Keywords

  • ELISA
  • exotoxin
  • immunodiagnosis
  • melioidiosis
  • Pseudomonas pseudomallei
  • serum agglutination

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Biochemistry
  • Plant Science
  • Food Science

Cite this

An ELISA-disc procedure for antibodies to Pseudomonas pseudomallei : application for a serological study of melioidosis in an endemic area. / Embi, Mohammed Noor; Devarajoo, D.; Mohamed, R.; Ismail, G.

In: World Journal of Microbiology & Biotechnology, Vol. 9, No. 1, 01.1993, p. 91-96.

Research output: Contribution to journalArticle

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