An assay for selection of sera with circulating toxoplasma gondii antigens

Emelia Osman, M. Zeehaida, O. Sulaiman, M. Rohela, G. Saadatnia, C. Yeng, N. Rahmah

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have developed an ELISA that employs monoclonal anti-Toxoplasma SAG1 (p30) as the capture antibody to detect T. gondii circulating antigens in patients' serum samples. Using serum spiked with Toxoplasma soluble and with SAG1 recombinant proteins, the detection limits were 31.25ng/mL and 62.50ng/mL, respectively. We obtained positive results in 28% (21/75) and 11% (23/206) of probable active and chronic toxoplasmosis serum samples, respectively. Western blot analysis on pooled antigen-positive serum samples showed antigenic bands of molecular weights 25 and 75kDa from sera of probable active infection and five antigenic bands ranging in size from 26 to 33kDa from chronic infection sera. This assay would be useful as an initial serum selection step in developing a Toxoplasma antigen detection test and for characterization studies.

Original languageEnglish
Pages (from-to)79-91
Number of pages13
JournalJournal of Immunoassay and Immunochemistry
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 2010
Externally publishedYes

Fingerprint

Toxoplasma
Assays
Antigens
Serum
Recombinant Proteins
Molecular weight
Antibodies
Toxoplasmosis
Infection
Limit of Detection
Molecular Weight
Western Blotting
Enzyme-Linked Immunosorbent Assay

Keywords

  • Circulating antigen
  • ELISA
  • Toxoplasma gondii
  • Western blot

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Biochemistry
  • Medical Laboratory Technology

Cite this

An assay for selection of sera with circulating toxoplasma gondii antigens. / Osman, Emelia; Zeehaida, M.; Sulaiman, O.; Rohela, M.; Saadatnia, G.; Yeng, C.; Rahmah, N.

In: Journal of Immunoassay and Immunochemistry, Vol. 31, No. 1, 01.2010, p. 79-91.

Research output: Contribution to journalArticle

Osman, E, Zeehaida, M, Sulaiman, O, Rohela, M, Saadatnia, G, Yeng, C & Rahmah, N 2010, 'An assay for selection of sera with circulating toxoplasma gondii antigens', Journal of Immunoassay and Immunochemistry, vol. 31, no. 1, pp. 79-91. https://doi.org/10.1080/15321810903405134
Osman, Emelia ; Zeehaida, M. ; Sulaiman, O. ; Rohela, M. ; Saadatnia, G. ; Yeng, C. ; Rahmah, N. / An assay for selection of sera with circulating toxoplasma gondii antigens. In: Journal of Immunoassay and Immunochemistry. 2010 ; Vol. 31, No. 1. pp. 79-91.
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