An amperometric biosensor for the rapid assessment of histamine level in tiger prawn (Penaeus monodon) spoilage

Ching Mai Keow, Fatimah Abu Bakar, Abu Bakar Salleh, Yook Heng Lee, Rahman Wagiran, Low Sim Bean

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Histamine could accumulate in seafood when bacteria spoilage commenced and caused histamine poisoning without altering the fish normal appearance and odor. Therefore, a histamine biosensor using immobilized enzyme diamine oxidase (DAO) has been developed for the rapid monitoring of the histamine levels in tiger prawn (Penaeus monodon). The histamine biosensor had a response time of <1 min and optimum pH of operation was 7.4 with reproducibility and repeatability (n = 5) of 4.87% and 5.26% relative standard deviations (RSD) respectively. Recoveries ranging from 93.11% to 100.58% were obtained for histamine spiked at levels from 5 to 20 ppm. The variation in histamine levels of some tiger prawn samples after a 5-h exposure at temperature of 30 °C ± 2 were studied using the histamine biosensor and the results were comparable to histamine levels determined by an HPLC method. The two methods showed a linear correlation with R2 = 0.9612 (Y = 0.9164x + 5.58). The limit of detection was 0.65 ppm of histamine, which is below the indicator level of 50 ppm established by USA FDA. The reusable biosensor is simple and can be used for direct histamine determination without further sample pretreatment, and is suitable for the routine analysis of histamine in tiger prawns to monitor spoilage.

Original languageEnglish
Pages (from-to)1636-1641
Number of pages6
JournalFood Chemistry
Volume105
Issue number4
DOIs
Publication statusPublished - 2007

Fingerprint

Penaeidae
Spoilage
Tigers
biosensors
Penaeus monodon
Biosensing Techniques
histamine
spoilage
Biosensors
Histamine
shrimp
scombroid poisoning
immobilized enzymes
Amine Oxidase (Copper-Containing)
Seafood
monitoring
Immobilized Enzymes
seafoods
Odors
reproducibility

Keywords

  • Amperometric biosensor
  • Diamine oxidase
  • Histamine
  • Tiger prawn (Penaeus monodon)

ASJC Scopus subject areas

  • Food Science

Cite this

An amperometric biosensor for the rapid assessment of histamine level in tiger prawn (Penaeus monodon) spoilage. / Keow, Ching Mai; Abu Bakar, Fatimah; Salleh, Abu Bakar; Lee, Yook Heng; Wagiran, Rahman; Bean, Low Sim.

In: Food Chemistry, Vol. 105, No. 4, 2007, p. 1636-1641.

Research output: Contribution to journalArticle

Keow, Ching Mai ; Abu Bakar, Fatimah ; Salleh, Abu Bakar ; Lee, Yook Heng ; Wagiran, Rahman ; Bean, Low Sim. / An amperometric biosensor for the rapid assessment of histamine level in tiger prawn (Penaeus monodon) spoilage. In: Food Chemistry. 2007 ; Vol. 105, No. 4. pp. 1636-1641.
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