An amperometric biosensor based on alanine dehydrogenase for the determination of low level of ammonium ion in water

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Abstract

An amperometric electrochemical biosensor has been developed for ammonium (NH 4 +) ion detection by immobilising alanine dehydrogenase (AlaDH) enzyme in a photocurable methacrylic membrane made up of poly(2-hydroxyethyl methacrylate) (pHEMA) on a screen-printed carbon paste electrode (SPE). The current detected was based on the electrocatalytic oxidation of nicotinamide adenine dinucleotide reduced (NADH) that is proportional to the consumption of NH 4 + ion whilst enzymatic amination of AlaDH and pyruvate is taking place. The biosensor was operated amperometrically at a potential of +0.6V and optimum pH 7. The NH 4 + biosensor demonstrated linear response to NH 4 + ion concentration in the range of 0.031.02mg/L with a limit of detection (LOD) of 8.52g/L. The proposed method has been successfully applied to the determination of NH 4 + ion in river water samples without any pretreatment. The levels of possible interferents in the waters were negligible to cause any interference on the proposed method. The analytical performance of the biosensor was comparable to the colorimetric method using Nesslerisation but with much lower detection limit and linear response range at ppb level.

Original languageEnglish
Article number980709
JournalJournal of Sensors
Volume2011
DOIs
Publication statusPublished - 2011

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dehydrogenases
alanine
bioinstrumentation
Biosensors
Ions
water
Water
ions
pyruvates
Amination
nicotinamide
adenines
ion concentration
pretreatment
rivers
enzymes
Enzymes
Rivers
membranes
Membranes

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering
  • Control and Systems Engineering

Cite this

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title = "An amperometric biosensor based on alanine dehydrogenase for the determination of low level of ammonium ion in water",
abstract = "An amperometric electrochemical biosensor has been developed for ammonium (NH 4 +) ion detection by immobilising alanine dehydrogenase (AlaDH) enzyme in a photocurable methacrylic membrane made up of poly(2-hydroxyethyl methacrylate) (pHEMA) on a screen-printed carbon paste electrode (SPE). The current detected was based on the electrocatalytic oxidation of nicotinamide adenine dinucleotide reduced (NADH) that is proportional to the consumption of NH 4 + ion whilst enzymatic amination of AlaDH and pyruvate is taking place. The biosensor was operated amperometrically at a potential of +0.6V and optimum pH 7. The NH 4 + biosensor demonstrated linear response to NH 4 + ion concentration in the range of 0.031.02mg/L with a limit of detection (LOD) of 8.52g/L. The proposed method has been successfully applied to the determination of NH 4 + ion in river water samples without any pretreatment. The levels of possible interferents in the waters were negligible to cause any interference on the proposed method. The analytical performance of the biosensor was comparable to the colorimetric method using Nesslerisation but with much lower detection limit and linear response range at ppb level.",
author = "{Tan @ Chong}, {Ling Ling} and Musa Ahmad and Lee, {Yook Heng}",
year = "2011",
doi = "10.1155/2011/980709",
language = "English",
volume = "2011",
journal = "Journal of Sensors",
issn = "1687-725X",
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AU - Lee, Yook Heng

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N2 - An amperometric electrochemical biosensor has been developed for ammonium (NH 4 +) ion detection by immobilising alanine dehydrogenase (AlaDH) enzyme in a photocurable methacrylic membrane made up of poly(2-hydroxyethyl methacrylate) (pHEMA) on a screen-printed carbon paste electrode (SPE). The current detected was based on the electrocatalytic oxidation of nicotinamide adenine dinucleotide reduced (NADH) that is proportional to the consumption of NH 4 + ion whilst enzymatic amination of AlaDH and pyruvate is taking place. The biosensor was operated amperometrically at a potential of +0.6V and optimum pH 7. The NH 4 + biosensor demonstrated linear response to NH 4 + ion concentration in the range of 0.031.02mg/L with a limit of detection (LOD) of 8.52g/L. The proposed method has been successfully applied to the determination of NH 4 + ion in river water samples without any pretreatment. The levels of possible interferents in the waters were negligible to cause any interference on the proposed method. The analytical performance of the biosensor was comparable to the colorimetric method using Nesslerisation but with much lower detection limit and linear response range at ppb level.

AB - An amperometric electrochemical biosensor has been developed for ammonium (NH 4 +) ion detection by immobilising alanine dehydrogenase (AlaDH) enzyme in a photocurable methacrylic membrane made up of poly(2-hydroxyethyl methacrylate) (pHEMA) on a screen-printed carbon paste electrode (SPE). The current detected was based on the electrocatalytic oxidation of nicotinamide adenine dinucleotide reduced (NADH) that is proportional to the consumption of NH 4 + ion whilst enzymatic amination of AlaDH and pyruvate is taking place. The biosensor was operated amperometrically at a potential of +0.6V and optimum pH 7. The NH 4 + biosensor demonstrated linear response to NH 4 + ion concentration in the range of 0.031.02mg/L with a limit of detection (LOD) of 8.52g/L. The proposed method has been successfully applied to the determination of NH 4 + ion in river water samples without any pretreatment. The levels of possible interferents in the waters were negligible to cause any interference on the proposed method. The analytical performance of the biosensor was comparable to the colorimetric method using Nesslerisation but with much lower detection limit and linear response range at ppb level.

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