An accurate and reliable method for identification and quantification of fatty acids and trans fatty acids in food fats samples using gas chromatography

Jumat Salimon, Talal A. Omar, Nadia Salih

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A method for the separation, identification and further quantification of fatty acids (FAs) and trans fatty acids (TFAs) by gas chromatography (GC) using the combination of lipid extraction and derivatization with the base-catalysed method followed by trimethylsilyl-diazomethane (TMS-DM) was developed. The proposed method was found to allow sensitive and accurate determination of a wide range of different types of FAs, including TFA isomers. The method was validated on real samples of dietary fat from hydrogenated edible oils (margarine) and nine standard FAs as representatives of margarines. For this purpose, response linearity, limit of detection (LOD), limit of quantification (LOQ), precision and recovery (R%) were all determined. Based on the results obtained, R-values from all the samples were revealed to be close to 100%, repeatability RSD ranged between 0.89% and 2.34%, and reproducibility RSD values ranged between 1.46% and 3.72%. The applicability of this method was demonstrated in four margarine samples and it was compared with the method used as reference. In general, the results proved that the proposed method is suitable for the analysis of FAs since it has shown higher effectiveness in TFA analysis than the classic methods. Thus, it could be an effective tool for analysing dietary fats and oils in complex mixtures of food products for the monitoring of low levels of FAs and TFA, and the control of labelling authenticity.

Original languageEnglish
JournalArabian Journal of Chemistry
DOIs
Publication statusAccepted/In press - 2013

Fingerprint

Trans Fatty Acids
Oils and fats
Fatty acids
Gas chromatography
Fats
Fatty Acids
Dietary Fats
Diazomethane
Unsaturated Dietary Fats
Complex Mixtures
Isomers
Labeling
Lipids
Recovery
Monitoring

Keywords

  • Derivatization
  • Food fats
  • Margarine
  • Quantification
  • Trans fatty acids

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

@article{5d0d6c27e93f4840927310755216dda6,
title = "An accurate and reliable method for identification and quantification of fatty acids and trans fatty acids in food fats samples using gas chromatography",
abstract = "A method for the separation, identification and further quantification of fatty acids (FAs) and trans fatty acids (TFAs) by gas chromatography (GC) using the combination of lipid extraction and derivatization with the base-catalysed method followed by trimethylsilyl-diazomethane (TMS-DM) was developed. The proposed method was found to allow sensitive and accurate determination of a wide range of different types of FAs, including TFA isomers. The method was validated on real samples of dietary fat from hydrogenated edible oils (margarine) and nine standard FAs as representatives of margarines. For this purpose, response linearity, limit of detection (LOD), limit of quantification (LOQ), precision and recovery (R{\%}) were all determined. Based on the results obtained, R-values from all the samples were revealed to be close to 100{\%}, repeatability RSD ranged between 0.89{\%} and 2.34{\%}, and reproducibility RSD values ranged between 1.46{\%} and 3.72{\%}. The applicability of this method was demonstrated in four margarine samples and it was compared with the method used as reference. In general, the results proved that the proposed method is suitable for the analysis of FAs since it has shown higher effectiveness in TFA analysis than the classic methods. Thus, it could be an effective tool for analysing dietary fats and oils in complex mixtures of food products for the monitoring of low levels of FAs and TFA, and the control of labelling authenticity.",
keywords = "Derivatization, Food fats, Margarine, Quantification, Trans fatty acids",
author = "Jumat Salimon and Omar, {Talal A.} and Nadia Salih",
year = "2013",
doi = "10.1016/j.arabjc.2013.07.016",
language = "English",
journal = "Arabian Journal of Chemistry",
issn = "1878-5352",
publisher = "King Saud University",

}

TY - JOUR

T1 - An accurate and reliable method for identification and quantification of fatty acids and trans fatty acids in food fats samples using gas chromatography

AU - Salimon, Jumat

AU - Omar, Talal A.

AU - Salih, Nadia

PY - 2013

Y1 - 2013

N2 - A method for the separation, identification and further quantification of fatty acids (FAs) and trans fatty acids (TFAs) by gas chromatography (GC) using the combination of lipid extraction and derivatization with the base-catalysed method followed by trimethylsilyl-diazomethane (TMS-DM) was developed. The proposed method was found to allow sensitive and accurate determination of a wide range of different types of FAs, including TFA isomers. The method was validated on real samples of dietary fat from hydrogenated edible oils (margarine) and nine standard FAs as representatives of margarines. For this purpose, response linearity, limit of detection (LOD), limit of quantification (LOQ), precision and recovery (R%) were all determined. Based on the results obtained, R-values from all the samples were revealed to be close to 100%, repeatability RSD ranged between 0.89% and 2.34%, and reproducibility RSD values ranged between 1.46% and 3.72%. The applicability of this method was demonstrated in four margarine samples and it was compared with the method used as reference. In general, the results proved that the proposed method is suitable for the analysis of FAs since it has shown higher effectiveness in TFA analysis than the classic methods. Thus, it could be an effective tool for analysing dietary fats and oils in complex mixtures of food products for the monitoring of low levels of FAs and TFA, and the control of labelling authenticity.

AB - A method for the separation, identification and further quantification of fatty acids (FAs) and trans fatty acids (TFAs) by gas chromatography (GC) using the combination of lipid extraction and derivatization with the base-catalysed method followed by trimethylsilyl-diazomethane (TMS-DM) was developed. The proposed method was found to allow sensitive and accurate determination of a wide range of different types of FAs, including TFA isomers. The method was validated on real samples of dietary fat from hydrogenated edible oils (margarine) and nine standard FAs as representatives of margarines. For this purpose, response linearity, limit of detection (LOD), limit of quantification (LOQ), precision and recovery (R%) were all determined. Based on the results obtained, R-values from all the samples were revealed to be close to 100%, repeatability RSD ranged between 0.89% and 2.34%, and reproducibility RSD values ranged between 1.46% and 3.72%. The applicability of this method was demonstrated in four margarine samples and it was compared with the method used as reference. In general, the results proved that the proposed method is suitable for the analysis of FAs since it has shown higher effectiveness in TFA analysis than the classic methods. Thus, it could be an effective tool for analysing dietary fats and oils in complex mixtures of food products for the monitoring of low levels of FAs and TFA, and the control of labelling authenticity.

KW - Derivatization

KW - Food fats

KW - Margarine

KW - Quantification

KW - Trans fatty acids

UR - http://www.scopus.com/inward/record.url?scp=84887339710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887339710&partnerID=8YFLogxK

U2 - 10.1016/j.arabjc.2013.07.016

DO - 10.1016/j.arabjc.2013.07.016

M3 - Article

JO - Arabian Journal of Chemistry

JF - Arabian Journal of Chemistry

SN - 1878-5352

ER -