Aminated hollow silica spheres for electrochemical DNA biosensor

Eda Yuhana Ariffin, Yook Heng Lee, Dedi Futra, Ling Ling Tan @ Chong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

An electrochemical DNA biosensor for e.coli determination based on aminated hollow silica was successfully developed. Aminated hollow silica spheres were prepared through the reaction of Tween 20 template and silica precursor. The template was removed by the thermal decomposition at 620°C. Hollow silica spheres were modified with (3-Aminopropyl) triethoxysilane (APTS) to form aminated hollow silica spheres.Aminated DNA probe were covalently immobilized on to the amine functionalized hollow silica spheres through glutaradehyde linkers. The formation hollow silica was characterized using FTIR and FESEM. A range of 50-300nm particle size obtained from FESEM micrograph. Meanwhile for the electrochemical study, a quasi-reversible system has been obtain via cyclic voltammetry (CV).

Original languageEnglish
Title of host publication2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Electronic)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Other

Other2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/4/1516/4/15

Fingerprint

bioinstrumentation
hollow
deoxyribonucleic acid
silicon dioxide
templates
thermal decomposition
amines
probes

Keywords

  • DNA biosensor
  • hollow silica

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Ariffin, E. Y., Lee, Y. H., Futra, D., & Tan @ Chong, L. L. (2015). Aminated hollow silica spheres for electrochemical DNA biosensor. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium (Vol. 1678). [050008] American Institute of Physics Inc.. https://doi.org/10.1063/1.4931287

Aminated hollow silica spheres for electrochemical DNA biosensor. / Ariffin, Eda Yuhana; Lee, Yook Heng; Futra, Dedi; Tan @ Chong, Ling Ling.

2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015. 050008.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ariffin, EY, Lee, YH, Futra, D & Tan @ Chong, LL 2015, Aminated hollow silica spheres for electrochemical DNA biosensor. in 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. vol. 1678, 050008, American Institute of Physics Inc., 2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015, Selangor, Malaysia, 15/4/15. https://doi.org/10.1063/1.4931287
Ariffin EY, Lee YH, Futra D, Tan @ Chong LL. Aminated hollow silica spheres for electrochemical DNA biosensor. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678. American Institute of Physics Inc. 2015. 050008 https://doi.org/10.1063/1.4931287
Ariffin, Eda Yuhana ; Lee, Yook Heng ; Futra, Dedi ; Tan @ Chong, Ling Ling. / Aminated hollow silica spheres for electrochemical DNA biosensor. 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015.
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