Altholactone, a novel styryl-lactone induces apoptosis via oxidative stress in human HL-60 leukemia cells

Salmaan H. Inayat-Hussain, Annuar Bin Osman, Laily Bin Din, Naoyuki Taniguchi

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    Plant styryl-lactone derivatives isolated from Goniothalamus sp. are potential compounds for cancer chemotherapy. In this study, we have examined the mechanisms of apoptosis induced by altholactone, a stryl-lactone isolated from the Malaysian plant G. malayanus on human HL-60 promyelocytic leukemia cells. Flow cytometric analysis of the externalization of phosphatidylserine (PS) using the annexin V/PI method on altholactone treated HL-60 cells showed a concentration-dependent increase of apoptosis from concentrations ranging from 10.8 (2.5 μg/ml) to 172.4 μM (40 μg/ml). Pre-treatment with the antioxidant N-acetylcysteine (1 mM) completely abrogated apoptosis induced by altholactone, suggesting for the involvement of oxidative stress. Further flow cytometric assessment of the level of intracellular peroxides using the fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCFH-DA) confirmed that altholactone induced an increase in cellular oxidative stress in HL-60 cells which was suppressed by N-acetylcysteine. In summary, our results demonstrate for the first time that altholactone induced apoptosis in HL-60 cells occurs via oxidative stress.

    Original languageEnglish
    Pages (from-to)153-159
    Number of pages7
    JournalToxicology Letters
    Volume131
    Issue number3
    DOIs
    Publication statusPublished - 28 May 2002

    Fingerprint

    Oxidative stress
    HL-60 Cells
    Lactones
    Leukemia
    Oxidative Stress
    Apoptosis
    Acetylcysteine
    Goniothalamus
    Chemotherapy
    Annexin A5
    Phosphatidylserines
    Peroxides
    Fluorescent Dyes
    Antioxidants
    altholactone
    Derivatives
    Drug Therapy
    Neoplasms

    Keywords

    • Altholactone
    • Apoptosis
    • N-acetylcysteine
    • Oxidative stress
    • Styryl-lactone

    ASJC Scopus subject areas

    • Toxicology

    Cite this

    Altholactone, a novel styryl-lactone induces apoptosis via oxidative stress in human HL-60 leukemia cells. / Inayat-Hussain, Salmaan H.; Osman, Annuar Bin; Din, Laily Bin; Taniguchi, Naoyuki.

    In: Toxicology Letters, Vol. 131, No. 3, 28.05.2002, p. 153-159.

    Research output: Contribution to journalArticle

    Inayat-Hussain, Salmaan H. ; Osman, Annuar Bin ; Din, Laily Bin ; Taniguchi, Naoyuki. / Altholactone, a novel styryl-lactone induces apoptosis via oxidative stress in human HL-60 leukemia cells. In: Toxicology Letters. 2002 ; Vol. 131, No. 3. pp. 153-159.
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