Alpha-tocopherol modulates hydrogen peroxide-induced DNA damage and telomere shortening of human skin fibroblasts derived from differently aged individuals

Suzana Makpol, Azalina Zainuddin, Norhazira Abdul Rahim, Yasmin Anum Mohd Yusof, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Antioxidants such as vitamin E may act differently on skin cells depending on the age of the skin and the level of oxidative damage induced. The effects of alpha-tocopherol (ATF) on Hinduced DNA damage and telomere shortening of normal human skin fibroblast cells derived from young and old individual donors were determined. Fibroblasts were divided into five groups; untreated control, Hinduced oxidative stress, alpha-tocopherol treatment, and pre-and post-treatment with alpha-tocopherol for Hinduced oxidative stress. Our results showed that Hinduced oxidative stress increased DNA damage, shortened the telomere length and reduced the telomerase activity (p<0.05) in fibroblasts obtained from young and old donors. Pre-and post-treatment with alpha-tocopherol protected against Hinduced DNA damage in fibroblasts obtained from young individuals (p=0.005; p=0.01, respectively). However, in fibroblasts obtained from old individuals, similar protective effects were only seen in cells pretreated with alpha-tocopherol (p=0.05) but not in the post-treated cells. Protection against Hinduced telomere shortening was observed in fibroblasts obtained from both young and old donors which were pre-treated with alpha-tocopherol (p=0.009; p=0.008, respectively). However, similar protective effects against telomere shortening in fibroblasts obtained from both young and old donors were not observed in the post-treated fibroblasts. Protection against Hinduced telomerase activity loss was observed only in fibroblasts obtained from old donors which were pretreated with alpha-tocopherol (p=0.04) but not in fibroblasts obtained from young donors. Similar protective effects against telomerase activity loss in fibroblasts obtained from both young and old donors were not observed in the post-treated fibroblasts. In conclusion, alpha-tocopherol protected against Hinduced telomere shortening by restoring the telomerase activity. It also modulated Hinduced DNA damage and this modulation was affected by donor age.

Original languageEnglish
Pages (from-to)869-875
Number of pages7
JournalPlanta Medica
Volume76
Issue number9
DOIs
Publication statusPublished - 2010

Fingerprint

Telomere Shortening
alpha-Tocopherol
Fibroblasts
Hydrogen Peroxide
DNA Damage
Skin
DNA
Telomerase
Oxidative stress
Oxidative Stress
Cells
Vitamin E

Keywords

  • aging
  • alphatocopherol
  • DNA damage
  • oxidative stress
  • telomerase
  • telomeres

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Molecular Medicine
  • Organic Chemistry
  • Analytical Chemistry
  • Pharmaceutical Science
  • Pharmacology
  • Drug Discovery

Cite this

Alpha-tocopherol modulates hydrogen peroxide-induced DNA damage and telomere shortening of human skin fibroblasts derived from differently aged individuals. / Makpol, Suzana; Zainuddin, Azalina; Rahim, Norhazira Abdul; Mohd Yusof, Yasmin Anum; Ngah, Wan Zurinah Wan.

In: Planta Medica, Vol. 76, No. 9, 2010, p. 869-875.

Research output: Contribution to journalArticle

Makpol, Suzana ; Zainuddin, Azalina ; Rahim, Norhazira Abdul ; Mohd Yusof, Yasmin Anum ; Ngah, Wan Zurinah Wan. / Alpha-tocopherol modulates hydrogen peroxide-induced DNA damage and telomere shortening of human skin fibroblasts derived from differently aged individuals. In: Planta Medica. 2010 ; Vol. 76, No. 9. pp. 869-875.
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AU - Makpol, Suzana

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AU - Mohd Yusof, Yasmin Anum

AU - Ngah, Wan Zurinah Wan

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N2 - Antioxidants such as vitamin E may act differently on skin cells depending on the age of the skin and the level of oxidative damage induced. The effects of alpha-tocopherol (ATF) on Hinduced DNA damage and telomere shortening of normal human skin fibroblast cells derived from young and old individual donors were determined. Fibroblasts were divided into five groups; untreated control, Hinduced oxidative stress, alpha-tocopherol treatment, and pre-and post-treatment with alpha-tocopherol for Hinduced oxidative stress. Our results showed that Hinduced oxidative stress increased DNA damage, shortened the telomere length and reduced the telomerase activity (p<0.05) in fibroblasts obtained from young and old donors. Pre-and post-treatment with alpha-tocopherol protected against Hinduced DNA damage in fibroblasts obtained from young individuals (p=0.005; p=0.01, respectively). However, in fibroblasts obtained from old individuals, similar protective effects were only seen in cells pretreated with alpha-tocopherol (p=0.05) but not in the post-treated cells. Protection against Hinduced telomere shortening was observed in fibroblasts obtained from both young and old donors which were pre-treated with alpha-tocopherol (p=0.009; p=0.008, respectively). However, similar protective effects against telomere shortening in fibroblasts obtained from both young and old donors were not observed in the post-treated fibroblasts. Protection against Hinduced telomerase activity loss was observed only in fibroblasts obtained from old donors which were pretreated with alpha-tocopherol (p=0.04) but not in fibroblasts obtained from young donors. Similar protective effects against telomerase activity loss in fibroblasts obtained from both young and old donors were not observed in the post-treated fibroblasts. In conclusion, alpha-tocopherol protected against Hinduced telomere shortening by restoring the telomerase activity. It also modulated Hinduced DNA damage and this modulation was affected by donor age.

AB - Antioxidants such as vitamin E may act differently on skin cells depending on the age of the skin and the level of oxidative damage induced. The effects of alpha-tocopherol (ATF) on Hinduced DNA damage and telomere shortening of normal human skin fibroblast cells derived from young and old individual donors were determined. Fibroblasts were divided into five groups; untreated control, Hinduced oxidative stress, alpha-tocopherol treatment, and pre-and post-treatment with alpha-tocopherol for Hinduced oxidative stress. Our results showed that Hinduced oxidative stress increased DNA damage, shortened the telomere length and reduced the telomerase activity (p<0.05) in fibroblasts obtained from young and old donors. Pre-and post-treatment with alpha-tocopherol protected against Hinduced DNA damage in fibroblasts obtained from young individuals (p=0.005; p=0.01, respectively). However, in fibroblasts obtained from old individuals, similar protective effects were only seen in cells pretreated with alpha-tocopherol (p=0.05) but not in the post-treated cells. Protection against Hinduced telomere shortening was observed in fibroblasts obtained from both young and old donors which were pre-treated with alpha-tocopherol (p=0.009; p=0.008, respectively). However, similar protective effects against telomere shortening in fibroblasts obtained from both young and old donors were not observed in the post-treated fibroblasts. Protection against Hinduced telomerase activity loss was observed only in fibroblasts obtained from old donors which were pretreated with alpha-tocopherol (p=0.04) but not in fibroblasts obtained from young donors. Similar protective effects against telomerase activity loss in fibroblasts obtained from both young and old donors were not observed in the post-treated fibroblasts. In conclusion, alpha-tocopherol protected against Hinduced telomere shortening by restoring the telomerase activity. It also modulated Hinduced DNA damage and this modulation was affected by donor age.

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