Allogeneic bilayered tissue-engineered skin promotes full-thickness wound healing in ovine model

Ruszymah Idrus, Mohd Adha Bin P Rameli, Low Kiat Cheong, Law Jia Xian, Chua Kien Hui, Mazlyzam Bin Abdul Latiff, Aminuddin Bin Saim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Skin injuries can be treated via grafting. However, since both autologous and allogeneic skin grafts have their own limitations, skin tissue engineering was developed as an alternative approach to promote healing. Autologous tissue-engineered skin requires a few weeks for cell culture and cannot be used for acute treatment. In this study, healing potential of allogeneic bilayered tissue-engineered skin (BTES) was evaluated in sheep. Isolated allogeneic skin cells from the sheep were cultured using a combined medium of DKSFM:F12:DMEM in the ratio 2:1:1 supplemented with 5% FBS. Differential trypsinization was later carried out to separate the fibroblasts and keratinocytes. The allogeneic BTES was fabricated using allogeneic fibrin as biomaterial by polymerizing the fibrin-keratinocyte layer with calcium chloride followed by fibrin-fibroblast layer. Half of the wounds were isolated with PVC rings to prevent cell migration. The wounds were treated with allogeneic BTES and silk, as control. Wounds were observed at days 7, 14 and 21 to determine the rate of reepithelialization. After 3 weeks, the sheep were euthanized. Histological evaluation with H & E, elastin van Gieson and Masson’s trichrome staining showed that allogeneic BTES treated wound healed faster compared to the control group. In conclusion, allogeneic BTES has the potential to be developed as off the shelf product for rapid treatment of full thickness wound.

Original languageEnglish
Pages (from-to)192-198
Number of pages7
JournalBiomedical Research
Volume25
Issue number2
Publication statusPublished - 2014

Fingerprint

Wound Healing
Sheep
Skin
Tissue
Wounds and Injuries
Fibrin
Fibroblasts
Keratinocytes
Calcium Chloride
Silk
Elastin
Biocompatible Materials
Tissue Engineering
Polyvinyl Chloride
Tissue engineering
Cell culture
Grafts
Cell Movement
Cell Culture Techniques
Staining and Labeling

Keywords

  • Allogeneic
  • Animal model
  • Bilayered tissue-engineered skin
  • Fibroblasts
  • Keratinocytes

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Idrus, R., Rameli, M. A. B. P., Cheong, L. K., Xian, L. J., Kien Hui, C., Latiff, M. B. A., & Saim, A. B. (2014). Allogeneic bilayered tissue-engineered skin promotes full-thickness wound healing in ovine model. Biomedical Research, 25(2), 192-198.

Allogeneic bilayered tissue-engineered skin promotes full-thickness wound healing in ovine model. / Idrus, Ruszymah; Rameli, Mohd Adha Bin P; Cheong, Low Kiat; Xian, Law Jia; Kien Hui, Chua; Latiff, Mazlyzam Bin Abdul; Saim, Aminuddin Bin.

In: Biomedical Research, Vol. 25, No. 2, 2014, p. 192-198.

Research output: Contribution to journalArticle

Idrus, R, Rameli, MABP, Cheong, LK, Xian, LJ, Kien Hui, C, Latiff, MBA & Saim, AB 2014, 'Allogeneic bilayered tissue-engineered skin promotes full-thickness wound healing in ovine model', Biomedical Research, vol. 25, no. 2, pp. 192-198.
Idrus, Ruszymah ; Rameli, Mohd Adha Bin P ; Cheong, Low Kiat ; Xian, Law Jia ; Kien Hui, Chua ; Latiff, Mazlyzam Bin Abdul ; Saim, Aminuddin Bin. / Allogeneic bilayered tissue-engineered skin promotes full-thickness wound healing in ovine model. In: Biomedical Research. 2014 ; Vol. 25, No. 2. pp. 192-198.
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