Transformasi gen proteolisis 6 (PRT6) berperantarakan agrobacterium tumefaciens ke dalam kotiledon tomato kultivar micro tom

Translated title of the contribution: Agrobacterium tumefaciens mediated transformation of the proteolysis 6 (PRT6) gene into cotyledons of tomato cv. Micro tom

Intan Elya Suka, Nur Farhana Roslan, Bee Lynn Chew, Hoe Han Goh, Zamri Zainal, Nurulhikma Md Isa

Research output: Contribution to journalArticle

Abstract

Proteolysis 6 (PRT6) gene plays an important role in the N-end rule pathway which functions as an E3 ligase enzyme. PRT6 functions to recognise target proteins for degradation. The main objective of this study is to transform the PRT6 RNAi construct through Agrobacterium tumefaciens into tomato. The purpose of this study was to understand the role of the N-end rule pathway during fruit ripening. Several factors affecting transformation efficiency such as co-cultivation time and concentration of antibiotics were optimised. The results from this study showed that pre-cultured cotyledons incubated for 48 h in co-cultivation medium increased the callus formation to 61% while using 500 mg/L carbenicillin antibiotic in the shoot regeneration medium reduced the contamination of A. tumefaciens to 5.2%. Besides, A. tumefaciens strain C58 was shown to be the most suitable A. tumefaciens strain to be used in this study. Polymerase Chain Reaction (PCR) was performed on the regenerated shoots to confirm integration of the PRT6 fragment into the tomato genome. Based on the PCR analysis, all putative transgenic shoots were positive transformants.

Original languageMalay
Pages (from-to)1465-1471
Number of pages7
JournalSains Malaysiana
Volume47
Issue number7
DOIs
Publication statusPublished - 1 Jul 2018

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Agrobacterium radiobacter
proteolysis
cotyledons
tomatoes
shoots
genes
polymerase chain reaction
antibiotics
carbenicillin
callus formation
protein degradation
ligases
ripening
genetically modified organisms
fruits
genome
enzymes

ASJC Scopus subject areas

  • General

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Transformasi gen proteolisis 6 (PRT6) berperantarakan agrobacterium tumefaciens ke dalam kotiledon tomato kultivar micro tom. / Suka, Intan Elya; Roslan, Nur Farhana; Chew, Bee Lynn; Goh, Hoe Han; Zainal, Zamri; Md Isa, Nurulhikma.

In: Sains Malaysiana, Vol. 47, No. 7, 01.07.2018, p. 1465-1471.

Research output: Contribution to journalArticle

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