Agrobacterium tumefaciens-mediated tranformation of chili with CMV (cucumber mosaic virus) coat protein gene

Nizam Kassim Sahidatul, Kalaivani K. Nadarajah, Ismail Bin Ahmad

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The recombinant plasmid, pSNC3301 carrying the coat protein gene of cucumber mosaic virus (CpCMV) was used in the Agrobacterium-mediated transformation of chili (Capsicum annuum L.). The transformation protocol required 2 days co-cultivation, 2 days pre-selection in non-BASTA™ herbicide containing medium, 3-4 weeks selection in BASTA™ herbicide containing medium, 20-25 weeks for culturing of adventitious shoot in regeneration medium and finally 1 week in roofing medium. PCR analysis detected the presence of CpCMV gene with transformation efficiency of 1.5% (7/467) for hypocotyl and 0.7% (4/536) for shoot tip. Histochemical GUS assay of hypocotyls, showed only nine from 22 PCR positive transformants showed GUS expression. For shoot tip, three of 13 PCR-positive transformants expressed GUS. The transformation efficiency of coat protein using pSNC3301 was 1.5% (7/467) for hypocotyls and 0.7% (4/536) for shoot tip.

Original languageEnglish
Pages (from-to)601-608
Number of pages8
JournalBiosciences Biotechnology Research Asia
Volume5
Issue number2
Publication statusPublished - Dec 2008

Fingerprint

Cucumovirus
Hypocotyl
Agrobacterium tumefaciens
Cucumber mosaic virus
Capsid Proteins
coat proteins
Agrobacterium radiobacter
hypocotyls
Herbicides
Polymerase Chain Reaction
herbicides
Genes
Capsicum
Agrobacterium
genes
adventitious shoots
Capsicum annuum
Regeneration
plasmids
Plasmids

Keywords

  • Agrobacterium coat protein
  • Capsicum annuum
  • CMV
  • Hypocotyls
  • Shoot tip
  • Transformation

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Drug Discovery

Cite this

Agrobacterium tumefaciens-mediated tranformation of chili with CMV (cucumber mosaic virus) coat protein gene. / Sahidatul, Nizam Kassim; K. Nadarajah, Kalaivani; Bin Ahmad, Ismail.

In: Biosciences Biotechnology Research Asia, Vol. 5, No. 2, 12.2008, p. 601-608.

Research output: Contribution to journalArticle

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