Age and gender effect on the growth of bone marrow stromal cells in vitro.

B. S. Shamsul, B. S. Aminuddin, Min Hwei Ng, Ruszymah Idrus

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Bone marrow harvested by aspiration contains connective tissue progenitor cells which can be selectively isolated and induced to express bone phenotype in vitro. The osteoblastic progenitor can be estimated by counting the number of cells attach using the haemacytometer. This study was undertaken to test the hypothesis that human aging is associated with a significant change on the number of osteoblastic progenitors in the bone marrow. Bone marrow aspirates were harvested from 38 patients, 14 men (age 11-70) and 24 women (age 10-70) and cultured in F12: DMEM (1:1). In total 15 bone marrow samples have been isolated from patients above 40 years old (men/women) of age. Fourteen (93.3%) of this samples failed to proliferate. Only one (6.7%) bone marrow sample from a male patient, aged 59 years old was successfully cultured. Seventy percent (16/23) of the samples from patient below than 40 years old were successfully cultured. However, our observation on the survival rate for cells of different gender from patient below 40 years old does not indicate any significant difference. From this study, we conclude that the growth of bone marrow stromal cells possibly for bone engineering is better from bone marrow aspirates of younger patient.

Original languageEnglish
Pages (from-to)196-197
Number of pages2
JournalThe Medical journal of Malaysia
Volume59 Suppl B
Publication statusPublished - May 2004

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Mesenchymal Stromal Cells
Bone Marrow
Growth
Connective Tissue Cells
Bone and Bones
In Vitro Techniques
Stem Cells
Survival Rate
Cell Count
Phenotype

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Age and gender effect on the growth of bone marrow stromal cells in vitro. / Shamsul, B. S.; Aminuddin, B. S.; Ng, Min Hwei; Idrus, Ruszymah.

In: The Medical journal of Malaysia, Vol. 59 Suppl B, 05.2004, p. 196-197.

Research output: Contribution to journalArticle

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