Advanced technologies for germplasm conservation of tropical fruit species

Rod Drew, Sarah Ashmore, Songpol Somsri, Normah Mohd. Noor, Tran Thi Hoa, Olivia Damasco, Ramanatha Rao

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Tropical fruit species are important worldwide and contribute to human nutrition, environmental protection and income generation. In the Asia, Pacific and Oceania region there are more than 400 tropical fruit species. However, many are subject to genetic erosion and their conservation is difficult or impossible by traditional methods due to seedlessness, recalcitrance, or short seed life. We developed conservation techniques, including in vitro protocols and cryopreservation, for major tropical fruit species in the collaborating countries. Protocols for vitrification-based shoot tip cryopreservation have been refined and applied to many papaya genotypes and Vasconcellea pubescens. Papaya seed has been stored for 12 months at a range of moisture contents and temperatures including cryostorage and was germinated after GA3 treatments. Somatic embryogenesis has been refined for mango and somatic embryos have been recovered after cryopreservation. Protocols for plantlet growth in vitro were achieved for two species of Nephelium. Shoot proliferation has been achieved for litchi and longan. Micropropagation and cryopreservation of embryogenic axes have been achieved for persimmon. Micropropagation protocols via adventitious shoot induction, microcutting or somatic embryogenesis were developed for seven Asian and three native Australian Citrus species. Seed/embryonic axes desiccation/ encapsulation-dehydration and vitrification of shoot tips/embryonic axes were employed for cryopreservation of citrus. Micropropagation and organogenesis have been achieved for three Australian native Davidsonia spp.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalActa Horticulturae
Volume760
Publication statusPublished - 2007

Fingerprint

tropical and subtropical fruits
germplasm conservation
cryopreservation
micropropagation
vitrification
papayas
somatic embryogenesis
Nephelium
Citrus
Vasconcellea pubescens
seeds
seedless varieties
Litchi
persimmons
Pacific Ocean Islands
environmental protection
human nutrition
adventitious shoots
encapsulation
organogenesis

Keywords

  • Carica
  • Citrus
  • Cryopreservation
  • Davidsonia
  • Mangifera
  • Nephelium
  • Vasconcellea

ASJC Scopus subject areas

  • Horticulture

Cite this

Drew, R., Ashmore, S., Somsri, S., Mohd. Noor, N., Hoa, T. T., Damasco, O., & Rao, R. (2007). Advanced technologies for germplasm conservation of tropical fruit species. Acta Horticulturae, 760, 91-98.

Advanced technologies for germplasm conservation of tropical fruit species. / Drew, Rod; Ashmore, Sarah; Somsri, Songpol; Mohd. Noor, Normah; Hoa, Tran Thi; Damasco, Olivia; Rao, Ramanatha.

In: Acta Horticulturae, Vol. 760, 2007, p. 91-98.

Research output: Contribution to journalArticle

Drew, R, Ashmore, S, Somsri, S, Mohd. Noor, N, Hoa, TT, Damasco, O & Rao, R 2007, 'Advanced technologies for germplasm conservation of tropical fruit species', Acta Horticulturae, vol. 760, pp. 91-98.
Drew R, Ashmore S, Somsri S, Mohd. Noor N, Hoa TT, Damasco O et al. Advanced technologies for germplasm conservation of tropical fruit species. Acta Horticulturae. 2007;760:91-98.
Drew, Rod ; Ashmore, Sarah ; Somsri, Songpol ; Mohd. Noor, Normah ; Hoa, Tran Thi ; Damasco, Olivia ; Rao, Ramanatha. / Advanced technologies for germplasm conservation of tropical fruit species. In: Acta Horticulturae. 2007 ; Vol. 760. pp. 91-98.
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