Adsorption of trypsin onto chitosan/Psf affinity membranes: Effects of physio-chemical environment

Sofiah Hamzah, Nora'aini Ali, Marinah Mohd Ariffin, Abdul Wahab Mohammad, Norasikin Othman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study aimed to investigate the significance of the physico-chemical environment during trypsin adsorption onto a highly bio-specific affinity membrane. The affinity matrix polysulfone (PSf) membranes were prepared via a simple dry/wet phase inversion technique. Surface modification of PSf membranes was employed using chitosan in order to improve membrane hydrophilicity. Glutaraldehyde and ovomucoid were used as the membrane activator and affinity ligand, respectively. Inspection of membrane morphology was done using scanning electron microscopy. The functional groups on the membrane surface were determined using Fourier-transform infrared spectroscopy equipped with attenuated total reflection (ATR-FTIR). In order to determine the optimum conditions for the maximum adsorption capacity of trypsin, adsorption studies were performed at different pH levels (5, 7, 8, 10, 12), ionic strengths (0.01, 0.05, 0.1, 0.3 and 0.5 M) and initial trypsin concentrations (0.1, 0.3, 0.5, 0.7 and 0.9 mg/ml). The optimum adsorption was obtained with a 0.9 mg/ml initial trypsin solution at pH 7 and an ionic strength of 0.1 M.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJurnal Teknologi
Volume74
Issue number7
DOIs
Publication statusPublished - 2015

Fingerprint

Chitosan
Membranes
Adsorption
Polysulfones
Ionic strength
Hydrophilicity
Functional groups
Fourier transform infrared spectroscopy
Surface treatment
Inspection
Ligands
Scanning electron microscopy

Keywords

  • Adsorption
  • Affinity
  • Chitosan
  • Membranes
  • Trypsin

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Adsorption of trypsin onto chitosan/Psf affinity membranes : Effects of physio-chemical environment. / Hamzah, Sofiah; Ali, Nora'aini; Ariffin, Marinah Mohd; Mohammad, Abdul Wahab; Othman, Norasikin.

In: Jurnal Teknologi, Vol. 74, No. 7, 2015, p. 1-6.

Research output: Contribution to journalArticle

Hamzah, Sofiah ; Ali, Nora'aini ; Ariffin, Marinah Mohd ; Mohammad, Abdul Wahab ; Othman, Norasikin. / Adsorption of trypsin onto chitosan/Psf affinity membranes : Effects of physio-chemical environment. In: Jurnal Teknologi. 2015 ; Vol. 74, No. 7. pp. 1-6.
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