Adenosine 3',5′-cyclic monophosphate extracted from local isolate of bacillus species

Tarik Mohammed Ali Rajab, Gaylany H. Abdullah, Hamid Ghaffoori Hasan, Abdul Amir H. Kadhum

Research output: Contribution to journalArticle

Abstract

Bacillus sp has been isolated locally from Sulaimani soil sample using nutrient agar plates. The isolate was identified as Bacillus genus according to the morphological feature of the colonies and cells. The bacterial growth was obtained using nutrient broth at 37 °C of pH 7.2. Adenosine 3',5′-cyclic monophosphate was extracted from the broth medium using incubation periods of 6, 12, 18 and 24 h. The extract was purified using Dowex-50 H from column chromatography technique. The purified adenosine 3',5′-cyclic monophosphate was identified using UV, HPLC and TLC analysis.

Original languageEnglish
Pages (from-to)4319-4322
Number of pages4
JournalAsian Journal of Chemistry
Volume25
Issue number8
Publication statusPublished - 2013

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Bacilli
Nutrients
Column chromatography
Agar
Soils
Cyclic AMP
Dowex 50

Keywords

  • Adenosine 3',5′-cyclic monophosphate
  • Bacillus species
  • Bacteria
  • Column chromatography

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Ali Rajab, T. M., Abdullah, G. H., Ghaffoori Hasan, H., & Kadhum, A. A. H. (2013). Adenosine 3',5′-cyclic monophosphate extracted from local isolate of bacillus species. Asian Journal of Chemistry, 25(8), 4319-4322.

Adenosine 3',5′-cyclic monophosphate extracted from local isolate of bacillus species. / Ali Rajab, Tarik Mohammed; Abdullah, Gaylany H.; Ghaffoori Hasan, Hamid; Kadhum, Abdul Amir H.

In: Asian Journal of Chemistry, Vol. 25, No. 8, 2013, p. 4319-4322.

Research output: Contribution to journalArticle

Ali Rajab, TM, Abdullah, GH, Ghaffoori Hasan, H & Kadhum, AAH 2013, 'Adenosine 3',5′-cyclic monophosphate extracted from local isolate of bacillus species', Asian Journal of Chemistry, vol. 25, no. 8, pp. 4319-4322.
Ali Rajab TM, Abdullah GH, Ghaffoori Hasan H, Kadhum AAH. Adenosine 3',5′-cyclic monophosphate extracted from local isolate of bacillus species. Asian Journal of Chemistry. 2013;25(8):4319-4322.
Ali Rajab, Tarik Mohammed ; Abdullah, Gaylany H. ; Ghaffoori Hasan, Hamid ; Kadhum, Abdul Amir H. / Adenosine 3',5′-cyclic monophosphate extracted from local isolate of bacillus species. In: Asian Journal of Chemistry. 2013 ; Vol. 25, No. 8. pp. 4319-4322.
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