Acyl homoserine lactonase genes from Bacillus species isolated from tomato rhizosphere soil in Malaysia

Noriha Mat Amin, Mohd Yusof Nor Rahim, Rohaiza Ahmad Redzuan, Amin Asyraf Tamizi, Bunawan Hamidun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Numerous gram negative bacterial plant pathogens use a cooperative cell to cell communication system known as quorum sensing to synchronize expression of genes essential for survival and virulence. N-Acyl-L-Homoserine Lactone (AHL) is the diffusible density-dependent signal molecule that is used in quorum sensing and is responsible for pathogenicity in most species of plant pathogenic bacteria including the papaya dieback disease pathogen, Erwinia mattotivora. Quorum quenching enzymes such as N-acyl-L-homoserine lactonase found in rhizosphere soil bacteria have the ability to degrade AHL and eliminate bacterial virulence therefore are key targets for disease control in plants. AHL inactivation genes coding for AHL lactonase were cloned from five Bacillus species isolated from tomato rhizosphere soil in Malaysia. Analysis of nucleotide sequences revealed an Open Reading Frame (ORF) of 753 bp which encodes a polypeptide of 250 amino acids with a predicted molecular weight of 28 kDa. BLAST analysis showed that the 753 bp nucleotide sequence was homologous with the AHL lactonase gene (aiiA) from Bacillus species in the GenBank database with 88-99% nucleotide sequence identity. The deduced AHL-lactonases have 90-100% amino acid identity with Bacillus aiiA proteins and belong to the group of metallo-beta-lactamase superfamily proteins.

Original languageEnglish
Pages (from-to)656-659
Number of pages4
JournalResearch Journal of Applied Sciences
Volume11
Issue number8
DOIs
Publication statusPublished - 2016

Fingerprint

Homoserine
tomatoes
Malaysia
Bacillus
nucleotides
Bacilli
Nucleotides
virulence
genes
soils
pathogens
Genes
Pathogens
Soils
bacteria
amino acids
Amino acids
Bacteria
Disease control
proteins

Keywords

  • AHL lactonase
  • Bacillus species
  • N-acyl-homoserine lactones
  • Quorum quenching
  • Quorum sensing

ASJC Scopus subject areas

  • Engineering(all)
  • General

Cite this

Acyl homoserine lactonase genes from Bacillus species isolated from tomato rhizosphere soil in Malaysia. / Amin, Noriha Mat; Rahim, Mohd Yusof Nor; Redzuan, Rohaiza Ahmad; Tamizi, Amin Asyraf; Hamidun, Bunawan.

In: Research Journal of Applied Sciences, Vol. 11, No. 8, 2016, p. 656-659.

Research output: Contribution to journalArticle

Amin, Noriha Mat ; Rahim, Mohd Yusof Nor ; Redzuan, Rohaiza Ahmad ; Tamizi, Amin Asyraf ; Hamidun, Bunawan. / Acyl homoserine lactonase genes from Bacillus species isolated from tomato rhizosphere soil in Malaysia. In: Research Journal of Applied Sciences. 2016 ; Vol. 11, No. 8. pp. 656-659.
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