Acute and subacute oral toxicity of Litsea elliptica Blume essential oil in rats

Siti Balkis Budin, Seri Masran Siti Nor Ain, Baharudin Omar, Taib Izatus Shima, Hidayatul Fathi Othman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Litsea elliptica Blume has been traditionally used to treat headache, fever, and stomach ulcer, and has also been used as an insect repellent. The acute and subacute toxicities of L. elliptica essential oil were evaluated orally by gavage in female Sprague-Dawley rats. For the acute toxicity study, L. elliptica essential oil was administered in doses from 500 to 4 000 mg/kg (single dose), and in the subacute toxicity test, the following doses were used: 125, 250, and 500 mg/kg, for 28 consecutive days. In the acute toxicity study, L. elliptica essential oil caused dose-dependent adverse behaviours and mortality. The median lethal dose value was 3 488.86 mg/kg and the acute non-observed-adversed-effect level value was found to be 500 mg/kg. The subacute toxicity study of L. elliptica essential oil did not reveal alterations in body weight, and food and water consumptions. The haematological and biochemical analyses did not show significant differences between control and treated groups in most of the parameters examined, except for the hemoglobin, mean cell hemoglobin concentration, mean cell volume, mean cell hemoglobin, serum albumin, and serum sodium. However, these differences were still within the normal range. No abnormalities or histopathological changes were observed in the liver, pancreatic islet of Langerhans, and renal glomerulous and tubular cells of all treated groups. In conclusion, L. elliptica essential oil can be classified in the U group, which is defined as a group unlikely to present an acute hazard according to World Health Organization (WHO) classification.

Original languageEnglish
Pages (from-to)783-790
Number of pages8
JournalJournal of Zhejiang University: Science B
Volume13
Issue number10
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Litsea
drug toxicity
Volatile Oils
Toxicity
subacute toxicity
Rats
essential oils
toxicity testing
acute toxicity
rats
hemoglobin
Hemoglobins
Erythrocyte Indices
islets of Langerhans
Islets of Langerhans
Subacute Toxicity Tests
Insect Repellents
dosage
cells
insect repellents

Keywords

  • Acute toxicity
  • Litsea elliptica
  • Median lethal dose (LD50)
  • Natural insecticide
  • Non-observed-adversed-effect level (NOAEL)
  • Subacute toxicity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • veterinary(all)

Cite this

Acute and subacute oral toxicity of Litsea elliptica Blume essential oil in rats. / Budin, Siti Balkis; Ain, Seri Masran Siti Nor; Omar, Baharudin; Izatus Shima, Taib; Othman, Hidayatul Fathi.

In: Journal of Zhejiang University: Science B, Vol. 13, No. 10, 10.2012, p. 783-790.

Research output: Contribution to journalArticle

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