Pengaktifan gen pelapor di dalam tumbuhan transgenik tomato melalui kaedah agro-infiltrasi vektor penandaan tanpa promoter

Translated title of the contribution: Activation of reporter gene in transgenic tomato by agro-infiltration of the promorterless tagging vector

Ahmad Safarina, Ismanizan Ismail, Zakaria Nurulfarhana, Zamri Zainal

Research output: Contribution to journalArticle

Abstract

A study on expiant inoculation method using promoterless tagging vector, pCAMDIN, on local tomato (MT1 variety) for the purpose of T-DNA insertional mutagenesis by agro-infiltration have been carried out. The injection of young leaves with a solution containing Agrobacterium tumefaciens, 100 mgll Str, 50 mgll Kan, 10mM MES and 100μM Acetosyringone. The infiltration was carried out with the use of LB medium by gently wounding the surface of the leaves and every procedures were carried out in the glass house. Infiltrated tissues were not damaged and remained green even after 5 days. Advantages of the technique included ease of infiltration and the observed transient expression on the sample, gus gene fragments were successfully amplified. Successful integration of gus gene into the tomato can also be observed in the results of Southern hybridization. Thus, it can be suggested that transgene insertion from recombinant vector pCAMDIN has occurred in tomato genome.

Original languageUndefined/Unknown
Pages (from-to)921-928
Number of pages8
JournalSains Malaysiana
Volume38
Issue number6
Publication statusPublished - Oct 2009

Fingerprint

agroinfiltration
reporter genes
genetically modified organisms
tomatoes
insertional mutagenesis
acetosyringone
inoculation methods
Agrobacterium radiobacter
Southern blotting
transgenes
leaves
genes
injection
greenhouses
genome
DNA
sampling
methodology

Keywords

  • A. Tumefaciens
  • Agro-infiltration
  • Transformation
  • Transgenic tomato

ASJC Scopus subject areas

  • General

Cite this

Pengaktifan gen pelapor di dalam tumbuhan transgenik tomato melalui kaedah agro-infiltrasi vektor penandaan tanpa promoter. / Safarina, Ahmad; Ismail, Ismanizan; Nurulfarhana, Zakaria; Zainal, Zamri.

In: Sains Malaysiana, Vol. 38, No. 6, 10.2009, p. 921-928.

Research output: Contribution to journalArticle

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abstract = "A study on expiant inoculation method using promoterless tagging vector, pCAMDIN, on local tomato (MT1 variety) for the purpose of T-DNA insertional mutagenesis by agro-infiltration have been carried out. The injection of young leaves with a solution containing Agrobacterium tumefaciens, 100 mgll Str, 50 mgll Kan, 10mM MES and 100μM Acetosyringone. The infiltration was carried out with the use of LB medium by gently wounding the surface of the leaves and every procedures were carried out in the glass house. Infiltrated tissues were not damaged and remained green even after 5 days. Advantages of the technique included ease of infiltration and the observed transient expression on the sample, gus gene fragments were successfully amplified. Successful integration of gus gene into the tomato can also be observed in the results of Southern hybridization. Thus, it can be suggested that transgene insertion from recombinant vector pCAMDIN has occurred in tomato genome.",
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AB - A study on expiant inoculation method using promoterless tagging vector, pCAMDIN, on local tomato (MT1 variety) for the purpose of T-DNA insertional mutagenesis by agro-infiltration have been carried out. The injection of young leaves with a solution containing Agrobacterium tumefaciens, 100 mgll Str, 50 mgll Kan, 10mM MES and 100μM Acetosyringone. The infiltration was carried out with the use of LB medium by gently wounding the surface of the leaves and every procedures were carried out in the glass house. Infiltrated tissues were not damaged and remained green even after 5 days. Advantages of the technique included ease of infiltration and the observed transient expression on the sample, gus gene fragments were successfully amplified. Successful integration of gus gene into the tomato can also be observed in the results of Southern hybridization. Thus, it can be suggested that transgene insertion from recombinant vector pCAMDIN has occurred in tomato genome.

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