Activated ADI pathway: The initiator of intermediate vancomycin resistance in Staphylococcus aureus

Xin Ee Tan, Hui Min Neoh, Mee Lee Looi, Siok Fong Chin, Longzhu Cui, Keiichi Hiramatsu, Salasawati Hussin, A. Rahman A. Jamal

Research output: Contribution to journalComment/debate

4 Citations (Scopus)

Abstract

Comparative proteomic profiling between 2 vancomycin-intermediate Staphylococcus aureus (VISA) strains, Mu50Ω-vraSm and Mu50Ω-vraSm-graRm, and vancomycin-susceptible S. aureus (VSSA) strain Mu50Ω revealed upregulated levels of catabolic ornithine carbamoyltransferase (ArcB) of the arginine catabolism pathway in VISA strains. Subsequent analyses showed that the VISA strains have higher levels of cellular ATP and ammonia, which are by-products of arginine catabolism, and displayed thicker cell walls. We postulate that elevated cytoplasmic ammonia and ATP molecules, resulting from activated arginine catabolism upon acquisition of vraS and graR mutations, are important requirements facilitating cell wall biosynthesis, thereby contributing to thickened cell wall and consequently reduced vancomycin susceptibility in VISA strains.

Original languageEnglish
Pages (from-to)260-264
Number of pages5
JournalCanadian Journal of Microbiology
Volume63
Issue number3
DOIs
Publication statusPublished - 2017

Fingerprint

Vancomycin Resistance
Vancomycin
Staphylococcus aureus
Cell Wall
Arginine
Ammonia
Adenosine Triphosphate
Ornithine Carbamoyltransferase
Proteomics
Mutation

Keywords

  • Ammonia
  • Arginine catabolism
  • ATP
  • Cell wall thickening
  • Vancomycin-intermediate Staphylococcus aureus

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

Cite this

Activated ADI pathway : The initiator of intermediate vancomycin resistance in Staphylococcus aureus. / Tan, Xin Ee; Neoh, Hui Min; Looi, Mee Lee; Chin, Siok Fong; Cui, Longzhu; Hiramatsu, Keiichi; Hussin, Salasawati; A. Jamal, A. Rahman.

In: Canadian Journal of Microbiology, Vol. 63, No. 3, 2017, p. 260-264.

Research output: Contribution to journalComment/debate

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AU - Chin, Siok Fong

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AU - Hiramatsu, Keiichi

AU - Hussin, Salasawati

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