Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection

Nur Fadhilah Mazlan, Ling Ling Tan @ Chong, Nurul Huda Abd Karim, Yook Heng Lee, Nur Diyana Jamaluddin, Nurul Yuziana Mohd Yusof, Doris Huai Xia Quay, Bahariah Khalid

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An optical genosensor based on Schiff base complex (Zn 2+ salphen) DNA label and acrylic microspheres (AMs) as polymer support of the capturing DNA probe (cpDNA) was developed for dengue virus serotype 2 (DEN-2) detection via reflectance spectrophotometric method. The solid-state optical DNA biosensor showed high selectivity and specificity up to one-base mismatch in the target DNA sequence owing to the salphen chemical structure that is rich in localized electrons, and allowed π-π stacking interaction between stacked base pairs of double-stranded DNA (dsDNA). The reflectometric DNA microsensor demonstrated a broad linear detection range towards DEN-2 DNA from 1 × 10 –15 M to 1 × 10 −3 M with a low limit of detection (LOD) obtained at 1.21 × 10 –16 M. The DNA biosensor gave reproducible optical response with a satisfactory relative standard deviation (RSD) at 3.1%, (n = 3), and the reflectance response was stable even after four regeneration cycles of the DNA biosensor. The optical genosensor was proven comparable with standard reverse transcription polymerase chain reaction (RT-PCR) in detecting DEN-2 genome acquired from clinical samples of serum, urine and saliva of dengue virus infected patients under informed consent. The developed reflectometric DNA biosensor is advantageous in offering an early DEN-2 diagnosis, when fever symptom started to manifest in patient.

Original languageEnglish
Pages (from-to)358-370
Number of pages13
JournalTalanta
Volume198
DOIs
Publication statusPublished - 1 Jun 2019

Fingerprint

Dengue Virus
Biosensing Techniques
Viruses
Biosensors
Labeling
Acrylics
Metals
Microsensors
Schiff Bases
Polymerase chain reaction
Transcription
Informed Consent
Microspheres
Saliva
Base Pairing
Reverse Transcription
Limit of Detection
Labels
Regeneration
Polymers

Keywords

  • Acrylic microsphere
  • Dengue virus
  • Optical biosensor
  • Reflectance spectrophotometer
  • Salphen

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry(all)
  • Biochemistry
  • Spectroscopy

Cite this

Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection. / Mazlan, Nur Fadhilah; Tan @ Chong, Ling Ling; Abd Karim, Nurul Huda; Lee, Yook Heng; Jamaluddin, Nur Diyana; Mohd Yusof, Nurul Yuziana; Quay, Doris Huai Xia; Khalid, Bahariah.

In: Talanta, Vol. 198, 01.06.2019, p. 358-370.

Research output: Contribution to journalArticle

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