Abstract
Skin and bone gelatins of pangasius catfish (Pangasius sutchi) were hydrolyzed with alcalase to isolate Angiotensin Converting Enzyme (ACE) inhibitory peptides. Samples with the highest degree of hydrolysis (DH) were separated into different fractions with molecular weight cut-off (MWCO) sizes of 10, 3 and 1 kDa, respectively and assayed for ACE inhibitory activity. Skin and bone gelatins had highest DH of 64.87 and 68.48 % after 2 and 1 h incubation, respectively. Results from this study indicated that by decreasing the molecular weight of fractions, ACE inhibitory activity was increased. Therefore, F3 permeates (MWCO < 1 kDa) of skin (IC50 = 3.2 μg/ml) and bone (IC50 = 1.3 μg/ml) gelatins possessed higher ACE inhibitory activity compared to their untreated gelatins and corresponding hydrolyzed fractions. In this study, the major amino acids were Glycine followed by Proline with an increased amount of hydrophobic amino acid content in F3 permeates of skin (4.01 %) and bone (5.79 %) gelatin. Digestion stability against gastrointestinal proteases did not show any remarkable change on ACE inhibition potency of these permeates. It was concluded that alcalase hydrolysis of P. sutchi by-products could be utilized as a part of functional food or ingredients of a formulated drug in order to control high blood pressure.
| Original language | English |
|---|---|
| Pages (from-to) | 1847-1856 |
| Number of pages | 10 |
| Journal | Journal of Food Science and Technology |
| Volume | 51 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2012 |
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Keywords
- ACE inhibitory
- Enzymatic hydrolysis
- Gelatin
- High blood pressure
- Pangasius sutchi
ASJC Scopus subject areas
- Food Science
Cite this
ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate. / Mahmoodani, Fatemeh; Ghassem, Masomeh; Babji, Abd. Salam; Mohamad Yusop, Salma; Khosrokhavar, Roya.
In: Journal of Food Science and Technology, Vol. 51, No. 9, 2012, p. 1847-1856.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate
AU - Mahmoodani, Fatemeh
AU - Ghassem, Masomeh
AU - Babji, Abd. Salam
AU - Mohamad Yusop, Salma
AU - Khosrokhavar, Roya
PY - 2012
Y1 - 2012
N2 - Skin and bone gelatins of pangasius catfish (Pangasius sutchi) were hydrolyzed with alcalase to isolate Angiotensin Converting Enzyme (ACE) inhibitory peptides. Samples with the highest degree of hydrolysis (DH) were separated into different fractions with molecular weight cut-off (MWCO) sizes of 10, 3 and 1 kDa, respectively and assayed for ACE inhibitory activity. Skin and bone gelatins had highest DH of 64.87 and 68.48 % after 2 and 1 h incubation, respectively. Results from this study indicated that by decreasing the molecular weight of fractions, ACE inhibitory activity was increased. Therefore, F3 permeates (MWCO < 1 kDa) of skin (IC50 = 3.2 μg/ml) and bone (IC50 = 1.3 μg/ml) gelatins possessed higher ACE inhibitory activity compared to their untreated gelatins and corresponding hydrolyzed fractions. In this study, the major amino acids were Glycine followed by Proline with an increased amount of hydrophobic amino acid content in F3 permeates of skin (4.01 %) and bone (5.79 %) gelatin. Digestion stability against gastrointestinal proteases did not show any remarkable change on ACE inhibition potency of these permeates. It was concluded that alcalase hydrolysis of P. sutchi by-products could be utilized as a part of functional food or ingredients of a formulated drug in order to control high blood pressure.
AB - Skin and bone gelatins of pangasius catfish (Pangasius sutchi) were hydrolyzed with alcalase to isolate Angiotensin Converting Enzyme (ACE) inhibitory peptides. Samples with the highest degree of hydrolysis (DH) were separated into different fractions with molecular weight cut-off (MWCO) sizes of 10, 3 and 1 kDa, respectively and assayed for ACE inhibitory activity. Skin and bone gelatins had highest DH of 64.87 and 68.48 % after 2 and 1 h incubation, respectively. Results from this study indicated that by decreasing the molecular weight of fractions, ACE inhibitory activity was increased. Therefore, F3 permeates (MWCO < 1 kDa) of skin (IC50 = 3.2 μg/ml) and bone (IC50 = 1.3 μg/ml) gelatins possessed higher ACE inhibitory activity compared to their untreated gelatins and corresponding hydrolyzed fractions. In this study, the major amino acids were Glycine followed by Proline with an increased amount of hydrophobic amino acid content in F3 permeates of skin (4.01 %) and bone (5.79 %) gelatin. Digestion stability against gastrointestinal proteases did not show any remarkable change on ACE inhibition potency of these permeates. It was concluded that alcalase hydrolysis of P. sutchi by-products could be utilized as a part of functional food or ingredients of a formulated drug in order to control high blood pressure.
KW - ACE inhibitory
KW - Enzymatic hydrolysis
KW - Gelatin
KW - High blood pressure
KW - Pangasius sutchi
UR - http://www.scopus.com/inward/record.url?scp=84920709132&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84920709132&partnerID=8YFLogxK
U2 - 10.1007/s13197-012-0742-8
DO - 10.1007/s13197-012-0742-8
M3 - Article
AN - SCOPUS:84920709132
VL - 51
SP - 1847
EP - 1856
JO - Journal of Food Science and Technology
JF - Journal of Food Science and Technology
SN - 0022-1155
IS - 9
ER -