ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate

Fatemeh Mahmoodani, Masomeh Ghassem, Abd. Salam Babji, Salma Mohamad Yusop, Roya Khosrokhavar

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Skin and bone gelatins of pangasius catfish (Pangasius sutchi) were hydrolyzed with alcalase to isolate Angiotensin Converting Enzyme (ACE) inhibitory peptides. Samples with the highest degree of hydrolysis (DH) were separated into different fractions with molecular weight cut-off (MWCO) sizes of 10, 3 and 1 kDa, respectively and assayed for ACE inhibitory activity. Skin and bone gelatins had highest DH of 64.87 and 68.48 % after 2 and 1 h incubation, respectively. Results from this study indicated that by decreasing the molecular weight of fractions, ACE inhibitory activity was increased. Therefore, F3 permeates (MWCO < 1 kDa) of skin (IC50 = 3.2 μg/ml) and bone (IC50 = 1.3 μg/ml) gelatins possessed higher ACE inhibitory activity compared to their untreated gelatins and corresponding hydrolyzed fractions. In this study, the major amino acids were Glycine followed by Proline with an increased amount of hydrophobic amino acid content in F3 permeates of skin (4.01 %) and bone (5.79 %) gelatin. Digestion stability against gastrointestinal proteases did not show any remarkable change on ACE inhibition potency of these permeates. It was concluded that alcalase hydrolysis of P. sutchi by-products could be utilized as a part of functional food or ingredients of a formulated drug in order to control high blood pressure.

Original languageEnglish
Pages (from-to)1847-1856
Number of pages10
JournalJournal of Food Science and Technology
Volume51
Issue number9
DOIs
Publication statusPublished - 2012

Fingerprint

Pangasius
Pangasianodon hypophthalmus
Catfishes
peptidyl-dipeptidase A
Peptidyl-Dipeptidase A
Gelatin
gelatin
hydrolysates
skin (animal)
catfish
bones
permeates
Bone and Bones
Skin
Subtilisins
Hydrolysis
subtilisin
hydrolysis
Molecular Weight
molecular weight

Keywords

  • ACE inhibitory
  • Enzymatic hydrolysis
  • Gelatin
  • High blood pressure
  • Pangasius sutchi

ASJC Scopus subject areas

  • Food Science

Cite this

ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate. / Mahmoodani, Fatemeh; Ghassem, Masomeh; Babji, Abd. Salam; Mohamad Yusop, Salma; Khosrokhavar, Roya.

In: Journal of Food Science and Technology, Vol. 51, No. 9, 2012, p. 1847-1856.

Research output: Contribution to journalArticle

Mahmoodani, Fatemeh ; Ghassem, Masomeh ; Babji, Abd. Salam ; Mohamad Yusop, Salma ; Khosrokhavar, Roya. / ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate. In: Journal of Food Science and Technology. 2012 ; Vol. 51, No. 9. pp. 1847-1856.
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