Accumulation of Biopesticide-Based Rotenone from an Optimized [BMIM][OTf] Green Binary Solvent Mixture in Different Parts of Terong Plant (Solanum Melongena)

Ho Yong Kang, Zetty Shafiqa Othman, Saiful Irwan Zubairi

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1 Citation (Scopus)

Abstract

Infestation of pests on the field crops has resulted losses on its productivity yearly. Moreover, over usage of pesticides has also affected consumer's health as pesticides residues are detected inside the post-harvested crops. Therefore, the aim of this study is to determine the accumulation of rotenone Derris sp. in leaves, stem and fruit of terong plant (Solanum melongena) through the degradation rate (k) and half-life (t1/2) of sprayed rotenone extract. The sprayed rotenone was prepared by using an optimized binary solvent mixture of 95% (v/v) acetone and ionic liquid (1-butyl-3-methylimidazolium trifluoromethansulfonate [BMIM] [OTf] via normal soaking extraction method. The same extraction method was used on the plant parts by using only 95% (v/v) of acetone. Reverse-phase high performance liquid chromatography (RP-HPLC) was used in analysing rotenone and [BMIM][OTf] traces. The results indicate that the degradation rates of 20 ppm were (1.35 ± 0.02 day-1 for leaves; 1.89 ± 0.07 day-1 for fruit) faster than 1000 ppm of rotenone extract (0.24 ± 0.00 day-1 for leaves; 0.21 ± 0.01 day-1 for stem; 0.31 ± 0.01 day-1 for fruit) (p<0.05) within 6 days of observation. Rotenone was identified to possess the half-life (t1/2) in the range of 0.37 ± 0.01 day to 0.51 ± 0.01 day in the study of 20 ppm rotenone extract. In contrast, the half-life (t1/2) for rotenone on 1000 ppm extract was in the range of 2.23 ± 0.05 days to 3.38 ± 0.10 days which was longer than 20 ppm extract (p<0.05). In conclusion, rotenone extract is still considered safe and has the potential to be used as a bio-pesticide due to its shorter half-life (t1/2) (less than 7 days at the highest concentration of 1000 ppm) than other commercial pesticides.

Original languageEnglish
Pages (from-to)702-709
Number of pages8
JournalProcedia Engineering
Volume148
DOIs
Publication statusPublished - 2016

Fingerprint

Pesticides
Fruits
Acetone
Crops
Degradation
High performance liquid chromatography
Ionic liquids
Productivity
Health

Keywords

  • biopesticide residual
  • Derris sp.
  • half life (t )
  • Rotenone
  • terong plant

ASJC Scopus subject areas

  • Engineering(all)

Cite this

@article{378e2a847fcd42e58e5b71729b40f840,
title = "Accumulation of Biopesticide-Based Rotenone from an Optimized [BMIM][OTf] Green Binary Solvent Mixture in Different Parts of Terong Plant (Solanum Melongena)",
abstract = "Infestation of pests on the field crops has resulted losses on its productivity yearly. Moreover, over usage of pesticides has also affected consumer's health as pesticides residues are detected inside the post-harvested crops. Therefore, the aim of this study is to determine the accumulation of rotenone Derris sp. in leaves, stem and fruit of terong plant (Solanum melongena) through the degradation rate (k) and half-life (t1/2) of sprayed rotenone extract. The sprayed rotenone was prepared by using an optimized binary solvent mixture of 95{\%} (v/v) acetone and ionic liquid (1-butyl-3-methylimidazolium trifluoromethansulfonate [BMIM] [OTf] via normal soaking extraction method. The same extraction method was used on the plant parts by using only 95{\%} (v/v) of acetone. Reverse-phase high performance liquid chromatography (RP-HPLC) was used in analysing rotenone and [BMIM][OTf] traces. The results indicate that the degradation rates of 20 ppm were (1.35 ± 0.02 day-1 for leaves; 1.89 ± 0.07 day-1 for fruit) faster than 1000 ppm of rotenone extract (0.24 ± 0.00 day-1 for leaves; 0.21 ± 0.01 day-1 for stem; 0.31 ± 0.01 day-1 for fruit) (p<0.05) within 6 days of observation. Rotenone was identified to possess the half-life (t1/2) in the range of 0.37 ± 0.01 day to 0.51 ± 0.01 day in the study of 20 ppm rotenone extract. In contrast, the half-life (t1/2) for rotenone on 1000 ppm extract was in the range of 2.23 ± 0.05 days to 3.38 ± 0.10 days which was longer than 20 ppm extract (p<0.05). In conclusion, rotenone extract is still considered safe and has the potential to be used as a bio-pesticide due to its shorter half-life (t1/2) (less than 7 days at the highest concentration of 1000 ppm) than other commercial pesticides.",
keywords = "biopesticide residual, Derris sp., half life (t ), Rotenone, terong plant",
author = "Kang, {Ho Yong} and Othman, {Zetty Shafiqa} and Zubairi, {Saiful Irwan}",
year = "2016",
doi = "10.1016/j.proeng.2016.06.578",
language = "English",
volume = "148",
pages = "702--709",
journal = "Procedia Engineering",
issn = "1877-7058",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Accumulation of Biopesticide-Based Rotenone from an Optimized [BMIM][OTf] Green Binary Solvent Mixture in Different Parts of Terong Plant (Solanum Melongena)

AU - Kang, Ho Yong

AU - Othman, Zetty Shafiqa

AU - Zubairi, Saiful Irwan

PY - 2016

Y1 - 2016

N2 - Infestation of pests on the field crops has resulted losses on its productivity yearly. Moreover, over usage of pesticides has also affected consumer's health as pesticides residues are detected inside the post-harvested crops. Therefore, the aim of this study is to determine the accumulation of rotenone Derris sp. in leaves, stem and fruit of terong plant (Solanum melongena) through the degradation rate (k) and half-life (t1/2) of sprayed rotenone extract. The sprayed rotenone was prepared by using an optimized binary solvent mixture of 95% (v/v) acetone and ionic liquid (1-butyl-3-methylimidazolium trifluoromethansulfonate [BMIM] [OTf] via normal soaking extraction method. The same extraction method was used on the plant parts by using only 95% (v/v) of acetone. Reverse-phase high performance liquid chromatography (RP-HPLC) was used in analysing rotenone and [BMIM][OTf] traces. The results indicate that the degradation rates of 20 ppm were (1.35 ± 0.02 day-1 for leaves; 1.89 ± 0.07 day-1 for fruit) faster than 1000 ppm of rotenone extract (0.24 ± 0.00 day-1 for leaves; 0.21 ± 0.01 day-1 for stem; 0.31 ± 0.01 day-1 for fruit) (p<0.05) within 6 days of observation. Rotenone was identified to possess the half-life (t1/2) in the range of 0.37 ± 0.01 day to 0.51 ± 0.01 day in the study of 20 ppm rotenone extract. In contrast, the half-life (t1/2) for rotenone on 1000 ppm extract was in the range of 2.23 ± 0.05 days to 3.38 ± 0.10 days which was longer than 20 ppm extract (p<0.05). In conclusion, rotenone extract is still considered safe and has the potential to be used as a bio-pesticide due to its shorter half-life (t1/2) (less than 7 days at the highest concentration of 1000 ppm) than other commercial pesticides.

AB - Infestation of pests on the field crops has resulted losses on its productivity yearly. Moreover, over usage of pesticides has also affected consumer's health as pesticides residues are detected inside the post-harvested crops. Therefore, the aim of this study is to determine the accumulation of rotenone Derris sp. in leaves, stem and fruit of terong plant (Solanum melongena) through the degradation rate (k) and half-life (t1/2) of sprayed rotenone extract. The sprayed rotenone was prepared by using an optimized binary solvent mixture of 95% (v/v) acetone and ionic liquid (1-butyl-3-methylimidazolium trifluoromethansulfonate [BMIM] [OTf] via normal soaking extraction method. The same extraction method was used on the plant parts by using only 95% (v/v) of acetone. Reverse-phase high performance liquid chromatography (RP-HPLC) was used in analysing rotenone and [BMIM][OTf] traces. The results indicate that the degradation rates of 20 ppm were (1.35 ± 0.02 day-1 for leaves; 1.89 ± 0.07 day-1 for fruit) faster than 1000 ppm of rotenone extract (0.24 ± 0.00 day-1 for leaves; 0.21 ± 0.01 day-1 for stem; 0.31 ± 0.01 day-1 for fruit) (p<0.05) within 6 days of observation. Rotenone was identified to possess the half-life (t1/2) in the range of 0.37 ± 0.01 day to 0.51 ± 0.01 day in the study of 20 ppm rotenone extract. In contrast, the half-life (t1/2) for rotenone on 1000 ppm extract was in the range of 2.23 ± 0.05 days to 3.38 ± 0.10 days which was longer than 20 ppm extract (p<0.05). In conclusion, rotenone extract is still considered safe and has the potential to be used as a bio-pesticide due to its shorter half-life (t1/2) (less than 7 days at the highest concentration of 1000 ppm) than other commercial pesticides.

KW - biopesticide residual

KW - Derris sp.

KW - half life (t )

KW - Rotenone

KW - terong plant

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JO - Procedia Engineering

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SN - 1877-7058

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