Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression

Sook Luan Ng, Anisah Nordin, Norzana Abd Ghafar, Yusof Suboh, Noraina Ab Rahim, Kien Hui Chua

Research output: Contribution to journalArticle

Abstract

Background: In recent years, the concern of Acanthamoeba keratitis has increased since the infection is often associated with contact lens use. Partial 18S rRNA genotypic identification of Acanthamoeba isolates is important to correlate with pathophysiological properties in order to evaluate the degree of virulence. This is the first report of genotypic identification for clinical isolates of Acanthamoeba from corneal scrapings of keratitis in Malaysia. This study is also the first to correlate the mRNA expression of MBP and AhLBP as virulent markers for axenic strains of Acanthamoeba. Results: In this study, ten clinical isolates were obtained from corneal scrapings. Rns genotype and intra-genotypic variation at the DF3 region of the isolates were identified. Results revealed that all clinical isolates belonged to the T4 genotype, with T4/6 (4 isolates), T4/2 (3 isolates), T4/16 (2 isolates) and one new genotype T4 sequence (T4/36), being determined. The axenic clinical isolates were cytopathogenic to rabbit corneal fibroblasts. MBP and AhLBP mRNA expression are directly correlated to Acanthamoeba cytopathic effect. Conclusions: All ten Malaysian clinical isolates were identified as genotype T4 which is predominantly associated with AK. Measuring the mRNA expression of Acanthamoeba virulent markers could be useful in the understanding of the pathogenesis of Acanthamoeba keratitis.

LanguageEnglish
Article number0625
JournalParasites and Vectors
Volume10
Issue number1
DOIs
StatePublished - 28 Dec 2017

Fingerprint

Acanthamoeba
Messenger RNA
Acanthamoeba Keratitis
Genotype
Keratitis
Malaysia
Contact Lenses
Virulence
Fibroblasts
Rabbits
Infection

Keywords

  • Acanthamoeba
  • AhLBP
  • Cytopathic
  • Genotype
  • Keratitis
  • MBP

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression. / Ng, Sook Luan; Nordin, Anisah; Abd Ghafar, Norzana; Suboh, Yusof; Ab Rahim, Noraina; Chua, Kien Hui.

In: Parasites and Vectors, Vol. 10, No. 1, 0625, 28.12.2017.

Research output: Contribution to journalArticle

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