Acacia honey modulates cell cycle progression, pro-inflammatory cytokines and calcium ions secretion in PC-3 cell lines

Muhammad Aliyu, Oyeronke A. Odunola, Ahsana D. Farooq, Osman Mesaik Mohammed Ahmed Hassan, Muhammad I. Choudhary, Beenish Fatima, Tariq A. Qureshi, Ochuko L. Erukainure

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Prostate cancer is the most common malignancy in men and the second leading cause of cancer-related deaths. Honey has been proven to have a potentiality of being among the functional foods by both in vivo and in vitro studies. Aim: This study was primarily designed to provide evidence on the apoptotic role of acacia honey on PC-3 cell line. Methods: MTT and mitotic index assay were used to determine the anti-proliferative effects. Cell cycle analysis was conducted on flow cytometer using propidium iodide. Expression of pro-inflammatory cytokines [TNF α and IL 1β] and prostate specific antigen were determined by ELISA kits. Calcium ion level was analyzed by colorimetric method. Results: Our results show the anti-proliferative effects of acacia honey on NIH/3T3 and PC-3 cell lines with the IC50 of 3.7 and 1.9% [v/v] with simultaneous increase on the secretion of calcium ion and down regulation of prostate specific antigen. Significant [p <0.05] dose-dependent modulation of G0/G1 phase was observed as compared with the control. However, there was an inverse relationship between TNF-α and IL-1β with latter being up regulated. Mitotic index of PC-3 cells decreased as the concentration of honey increases. Conclusion: Apoptotic role of acacia honey on PC-3 Cell line may be due to modulation of G0/G1 phase, Proinflammatory Cytokines, Calcium ions secretion and down regulation of Prostate specific antigen in vitro.

Original languageEnglish
Pages (from-to)401-407
Number of pages7
JournalJournal of Cancer Science and Therapy
Volume4
Issue number12
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Acacia
Honey
Cell Cycle
Ions
Cytokines
Calcium
Cell Line
Prostate-Specific Antigen
Cell Cycle Resting Phase
Mitotic Index
G1 Phase
Interleukin-1
Down-Regulation
Functional Food
Propidium
Inhibitory Concentration 50
Neoplasms
Prostatic Neoplasms
Enzyme-Linked Immunosorbent Assay

Keywords

  • Acacia honey
  • Apoptosis
  • Calcium ion
  • Cytokines
  • PC-3 cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Aliyu, M., Odunola, O. A., Farooq, A. D., Mohammed Ahmed Hassan, O. M., Choudhary, M. I., Fatima, B., ... Erukainure, O. L. (2012). Acacia honey modulates cell cycle progression, pro-inflammatory cytokines and calcium ions secretion in PC-3 cell lines. Journal of Cancer Science and Therapy, 4(12), 401-407. https://doi.org/10.4172/1948-5956.1000174

Acacia honey modulates cell cycle progression, pro-inflammatory cytokines and calcium ions secretion in PC-3 cell lines. / Aliyu, Muhammad; Odunola, Oyeronke A.; Farooq, Ahsana D.; Mohammed Ahmed Hassan, Osman Mesaik; Choudhary, Muhammad I.; Fatima, Beenish; Qureshi, Tariq A.; Erukainure, Ochuko L.

In: Journal of Cancer Science and Therapy, Vol. 4, No. 12, 2012, p. 401-407.

Research output: Contribution to journalArticle

Aliyu, M, Odunola, OA, Farooq, AD, Mohammed Ahmed Hassan, OM, Choudhary, MI, Fatima, B, Qureshi, TA & Erukainure, OL 2012, 'Acacia honey modulates cell cycle progression, pro-inflammatory cytokines and calcium ions secretion in PC-3 cell lines', Journal of Cancer Science and Therapy, vol. 4, no. 12, pp. 401-407. https://doi.org/10.4172/1948-5956.1000174
Aliyu, Muhammad ; Odunola, Oyeronke A. ; Farooq, Ahsana D. ; Mohammed Ahmed Hassan, Osman Mesaik ; Choudhary, Muhammad I. ; Fatima, Beenish ; Qureshi, Tariq A. ; Erukainure, Ochuko L. / Acacia honey modulates cell cycle progression, pro-inflammatory cytokines and calcium ions secretion in PC-3 cell lines. In: Journal of Cancer Science and Therapy. 2012 ; Vol. 4, No. 12. pp. 401-407.
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abstract = "Background: Prostate cancer is the most common malignancy in men and the second leading cause of cancer-related deaths. Honey has been proven to have a potentiality of being among the functional foods by both in vivo and in vitro studies. Aim: This study was primarily designed to provide evidence on the apoptotic role of acacia honey on PC-3 cell line. Methods: MTT and mitotic index assay were used to determine the anti-proliferative effects. Cell cycle analysis was conducted on flow cytometer using propidium iodide. Expression of pro-inflammatory cytokines [TNF α and IL 1β] and prostate specific antigen were determined by ELISA kits. Calcium ion level was analyzed by colorimetric method. Results: Our results show the anti-proliferative effects of acacia honey on NIH/3T3 and PC-3 cell lines with the IC50 of 3.7 and 1.9{\%} [v/v] with simultaneous increase on the secretion of calcium ion and down regulation of prostate specific antigen. Significant [p <0.05] dose-dependent modulation of G0/G1 phase was observed as compared with the control. However, there was an inverse relationship between TNF-α and IL-1β with latter being up regulated. Mitotic index of PC-3 cells decreased as the concentration of honey increases. Conclusion: Apoptotic role of acacia honey on PC-3 Cell line may be due to modulation of G0/G1 phase, Proinflammatory Cytokines, Calcium ions secretion and down regulation of Prostate specific antigen in vitro.",
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AU - Mohammed Ahmed Hassan, Osman Mesaik

AU - Choudhary, Muhammad I.

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