Abnormality in DNA-protein binding in ataxia-telangiectasia nuclear extracts

Rahmah Mohamed, Martin F. Lavin

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Anomalies in DNA replication, repair and recombination in ataxia-telangiectasia (A-T) point to a defect in structure or function of chromatin. In this study we have compared DNA-protein binding in nuclear extracts from control and A-T cells using two assay systems, filter-binding and DNA-accessibility. Interestingly, the extent of DNA protein binding over a range of protein concentration was significantly lower in A-T extracts. In addition the accessibility of the restriction enzyme Eco R1 to protein-bound plasmid was greater when A-T extracts were used. This is in keeping with the reduced binding observed in the filter-binding assay.

    Original languageEnglish
    Pages (from-to)749-754
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume158
    Issue number3
    DOIs
    Publication statusPublished - 15 Feb 1989

    Fingerprint

    Ataxia Telangiectasia
    DNA-Binding Proteins
    Assays
    T-cells
    DNA
    DNA Replication
    DNA Repair
    Genetic Recombination
    Chromatin
    Proteins
    Plasmids
    Repair
    T-Lymphocytes
    Defects
    Enzymes

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Abnormality in DNA-protein binding in ataxia-telangiectasia nuclear extracts. / Mohamed, Rahmah; Lavin, Martin F.

    In: Biochemical and Biophysical Research Communications, Vol. 158, No. 3, 15.02.1989, p. 749-754.

    Research output: Contribution to journalArticle

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