Abnormality and mortality of irradiated immature stages of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera

Tephritidae) by gamma irradiation

Suhana Yusof, Ahmad Zainuri, Salmah Yaakop

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is one of the most important quarantine pests in the world. Low dose of gamma radiation was proven as possible phytosanitary treatment to disinfest this pest to permit exports to other countries. Treatments was conducted in Malaysian Nuclear Agency, Dengkil, Selangor using a Cesium cell-137 source (Gamma Cell Biobeam GM 8000). The objectives of this study are to investigate the percentage of mortality, emergence and malformation rate of irradiated all instar larvae and pupae of Bactrocera dorsalis at various dosages of gamma radiation. Samples of immature stages of flies were tested separately with nine radiation dose of 0, 50, 100, 150, 200, 250, 300, 350 and 400Gy. The result shows that 100% mortality of treated 1st instar was determined at dose 250Gy but all treated doses were not able to prevent pupation from irradiated 2nd instar and 3rd instar larvae, even at the highest dose, 400Gray. At dose 150 Gray and above all the survived pupae from irradiated 2nd and 3rd larvae were completely dead and failed to emerge as an adult. Although all treated doses were not able to prevent adult emergence from treated pupae, but result shows in inverse proportion of emergence rate with dosages (when the dose increases, % adult eclosion decreased). Radiation dose start from 150Gy and above also affected the malformation rates of pupae and adult by increased significantly when the dose of the radiation increases. In conclusion, at dose 150Gy which give efficacy of inhibition of adult emergence of 3rd instar larvae and pupae is suggested as a possible minimum dose for phytosanitary treatment of Bactrocera dorsalis fruit fly. However, before the appropriate dose of irradiation treatment for quarantine security can be recommended, more information is needed on the effect of irradiation doses on sterility, fecundity, and genetic changes of Bactrocera dorsalis.

Original languageEnglish
Title of host publication2018 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium
EditorsNoor Hayati Ahmad Rasol, Kamarulzaman Ibrahim, Siti Aishah Hasbullah, Mohammad Hafizuddin Hj. Jumali, Nazlina Ibrahim, Marlia Mohd Hanafiah, Mohd Talib Latif
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418431
DOIs
Publication statusPublished - 27 Jun 2019
Event2018 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 4 Apr 20186 Apr 2018

Publication series

NameAIP Conference Proceedings
Volume2111
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2018 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period4/4/186/4/18

Fingerprint

Bactrocera dorsalis
fruits
mortality
abnormalities
Tephritidae
abnormality
gamma radiation
irradiation
fruit
immatures
dosage
pupa
larvae
pupae
eclosion
instars
larva
quarantine
dose
pupation

Keywords

  • Abnormality
  • Bactrocera dorsalis
  • gamma irradiation
  • mortality

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science
  • Physics and Astronomy(all)
  • Nature and Landscape Conservation

Cite this

Yusof, S., Zainuri, A., & Yaakop, S. (2019). Abnormality and mortality of irradiated immature stages of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) by gamma irradiation. In N. H. A. Rasol, K. Ibrahim, S. A. Hasbullah, M. H. H. Jumali, N. Ibrahim, M. M. Hanafiah, & M. T. Latif (Eds.), 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium [060022] (AIP Conference Proceedings; Vol. 2111). American Institute of Physics Inc.. https://doi.org/10.1063/1.5111284

Abnormality and mortality of irradiated immature stages of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera : Tephritidae) by gamma irradiation. / Yusof, Suhana; Zainuri, Ahmad; Yaakop, Salmah.

2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. ed. / Noor Hayati Ahmad Rasol; Kamarulzaman Ibrahim; Siti Aishah Hasbullah; Mohammad Hafizuddin Hj. Jumali; Nazlina Ibrahim; Marlia Mohd Hanafiah; Mohd Talib Latif. American Institute of Physics Inc., 2019. 060022 (AIP Conference Proceedings; Vol. 2111).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yusof, S, Zainuri, A & Yaakop, S 2019, Abnormality and mortality of irradiated immature stages of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) by gamma irradiation. in NHA Rasol, K Ibrahim, SA Hasbullah, MHH Jumali, N Ibrahim, MM Hanafiah & MT Latif (eds), 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium., 060022, AIP Conference Proceedings, vol. 2111, American Institute of Physics Inc., 2018 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 4/4/18. https://doi.org/10.1063/1.5111284
Yusof S, Zainuri A, Yaakop S. Abnormality and mortality of irradiated immature stages of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) by gamma irradiation. In Rasol NHA, Ibrahim K, Hasbullah SA, Jumali MHH, Ibrahim N, Hanafiah MM, Latif MT, editors, 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. American Institute of Physics Inc. 2019. 060022. (AIP Conference Proceedings). https://doi.org/10.1063/1.5111284
Yusof, Suhana ; Zainuri, Ahmad ; Yaakop, Salmah. / Abnormality and mortality of irradiated immature stages of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera : Tephritidae) by gamma irradiation. 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. editor / Noor Hayati Ahmad Rasol ; Kamarulzaman Ibrahim ; Siti Aishah Hasbullah ; Mohammad Hafizuddin Hj. Jumali ; Nazlina Ibrahim ; Marlia Mohd Hanafiah ; Mohd Talib Latif. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).
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abstract = "The Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is one of the most important quarantine pests in the world. Low dose of gamma radiation was proven as possible phytosanitary treatment to disinfest this pest to permit exports to other countries. Treatments was conducted in Malaysian Nuclear Agency, Dengkil, Selangor using a Cesium cell-137 source (Gamma Cell Biobeam GM 8000). The objectives of this study are to investigate the percentage of mortality, emergence and malformation rate of irradiated all instar larvae and pupae of Bactrocera dorsalis at various dosages of gamma radiation. Samples of immature stages of flies were tested separately with nine radiation dose of 0, 50, 100, 150, 200, 250, 300, 350 and 400Gy. The result shows that 100{\%} mortality of treated 1st instar was determined at dose 250Gy but all treated doses were not able to prevent pupation from irradiated 2nd instar and 3rd instar larvae, even at the highest dose, 400Gray. At dose 150 Gray and above all the survived pupae from irradiated 2nd and 3rd larvae were completely dead and failed to emerge as an adult. Although all treated doses were not able to prevent adult emergence from treated pupae, but result shows in inverse proportion of emergence rate with dosages (when the dose increases, {\%} adult eclosion decreased). Radiation dose start from 150Gy and above also affected the malformation rates of pupae and adult by increased significantly when the dose of the radiation increases. In conclusion, at dose 150Gy which give efficacy of inhibition of adult emergence of 3rd instar larvae and pupae is suggested as a possible minimum dose for phytosanitary treatment of Bactrocera dorsalis fruit fly. However, before the appropriate dose of irradiation treatment for quarantine security can be recommended, more information is needed on the effect of irradiation doses on sterility, fecundity, and genetic changes of Bactrocera dorsalis.",
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