A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion

Siti Nur Syazni Mohd Zuki, Ling Ling Tan @ Chong, Nina Suhaity Azmi, Yook Heng Lee, Kwok Feng Chong, Saiful Nizam Tajuddin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A microspheres-based microbial optosensor for NO2 - ion quantitation was constructed by using immobilized Raoutella planticola (R. planticola), the bacterium expressing NAD(P)H nitrite reductase (NiR) enzyme, which was isolated from local edible bird's nest (EBN) via microbial technique. The whole cells and the lipophilic Nile Blue chromoionophore (NBC) were physically adsorbed on the self-adhesive photocurable poly(n-butyl acrylate-co-N-acryloxysuccinimide) [poly(nBA-NAS)] microspheres, whilst the reduced co-enzyme NAD(P)H was covalently immobilized on the succinimide-functionalized acrylic microspheres via peptide link to produce a reagentless nitrite biosensing system. As the microbial bio-optode responded to nitrite through colour change from blue to pink, a facile reflectometric approach was adopted to measure reflectance intensity at 639nm, before and after reaction with nitrite at optimum pH 8. The optosensor could quantify NO2 - ion concentration within a dynamic linear response range of 0.5-400mgL-1 with a limit of detection (LOD) of 0.2mgL-1. The large surface area to volume ratio of the acrylic microspheres allowed solid-state diffusional mass transfer of the substrate to occur at micro-bio-optode surface, and an equilibrium response was achieved within 5min. The practical feasibility of using the bio-optode for nitrite assay in food matrix sample showed good agreement with standard ion chromatography method.

Original languageEnglish
JournalSensors and Actuators, B: Chemical
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

nitrites
microorganisms
Nitrites
Microspheres
Microorganisms
Acrylics
Ions
cells
Nitrite Reductase (NAD(P)H)
ions
Enzymes
enzymes
Ion chromatography
succinimides
Birds
birds
NAD
Peptides
Assays
chromatography

Keywords

  • Edible bird's nest
  • Nitrite
  • Optode
  • Raoutella planticola
  • Reflectance

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry
  • Electrical and Electronic Engineering

Cite this

A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion. / Mohd Zuki, Siti Nur Syazni; Tan @ Chong, Ling Ling; Azmi, Nina Suhaity; Lee, Yook Heng; Chong, Kwok Feng; Tajuddin, Saiful Nizam.

In: Sensors and Actuators, B: Chemical, 2017.

Research output: Contribution to journalArticle

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abstract = "A microspheres-based microbial optosensor for NO2 - ion quantitation was constructed by using immobilized Raoutella planticola (R. planticola), the bacterium expressing NAD(P)H nitrite reductase (NiR) enzyme, which was isolated from local edible bird's nest (EBN) via microbial technique. The whole cells and the lipophilic Nile Blue chromoionophore (NBC) were physically adsorbed on the self-adhesive photocurable poly(n-butyl acrylate-co-N-acryloxysuccinimide) [poly(nBA-NAS)] microspheres, whilst the reduced co-enzyme NAD(P)H was covalently immobilized on the succinimide-functionalized acrylic microspheres via peptide link to produce a reagentless nitrite biosensing system. As the microbial bio-optode responded to nitrite through colour change from blue to pink, a facile reflectometric approach was adopted to measure reflectance intensity at 639nm, before and after reaction with nitrite at optimum pH 8. The optosensor could quantify NO2 - ion concentration within a dynamic linear response range of 0.5-400mgL-1 with a limit of detection (LOD) of 0.2mgL-1. The large surface area to volume ratio of the acrylic microspheres allowed solid-state diffusional mass transfer of the substrate to occur at micro-bio-optode surface, and an equilibrium response was achieved within 5min. The practical feasibility of using the bio-optode for nitrite assay in food matrix sample showed good agreement with standard ion chromatography method.",
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