A validation method development for simultaneous LC-ESI-TOF/MS analysis of some pharmaceuticals in tangkas river-Malaysia

Fouad F. Al-Qaim, Md. Pauzi Abdullah, Mohamed Rozali Othman, Jalifah Latip, Wan Afiq

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Pharmaceuticals are synthetic or natural chemicals that can be found in prescription medicines, over-the-counter therapeutic drugs and veterinary drugs. The occurrence of pharmaceuticals in the environment and the water cycle at trace levels (in the range of nanograms to low micrograms per litre) has been widely discussed and published in literature in the past decade. The increase in detection is largely attributable to the advances in analytical techniques and instrumentation. This paper describes development, optimisation and validation of a method for the simultaneous analysis of 7 multi class pharmaceuticals (caffeine (CAF), Prazosin (PRZ), enalapril maleate (ENL), carbamazepine (CBZ), nifedipine (NFD), levonorgestrel (LNG), simvastatin (SMV)) using solid phase extraction (SPE cartridges Oasis HLB) followed by liquid chromatography-time-of- flight/mass spectrometry (LC ESI TOF/MS). Its linearity, 0.5-250 μg L-1, provided determination coefficients (R2) above 0.99 for all compounds. The Instrumental Quantification Limits (IQLs) for all pharmaceuticals ranged from 0.5-5 μg L-1 in the solvent as a standard mixture (i.e., direct injection). The extraction efficiency (EE%) was more than 40% and 60% in river and deionised water for most of compounds, respectively. Limit of quantification (LOQ) for all pharmaceuticals ranged from 13-800 ng L -1 in spiked river water. The inter and intra day precision of the method, calculated as the relative standard deviation (RSD%), 2.33-22.3% and 0.6-9.9%, respectively, except for caffeine, which has a RSD% of 20.1% at 50 μg L-1. Matrix effect was investigated and ranged from 10-41%. Out of seven pharmaceuticals, six pharmaceutical compounds were detected in the river water sample.

Original languageEnglish
Pages (from-to)271-281
Number of pages11
JournalJournal of the Brazilian Chemical Society
Volume25
Issue number2
DOIs
Publication statusPublished - Feb 2014

Fingerprint

Rivers
Pharmaceutical Preparations
Caffeine
Water
Veterinary Drugs
Levonorgestrel
Enalapril
Simvastatin
Deionized water
Prazosin
Liquid chromatography
Carbamazepine
Direct injection
Nifedipine
Mass spectrometry

Keywords

  • Basic pharmaceuticals
  • Extraction efficiency
  • LC-ESI-TOF/MS
  • Solid phase extraction
  • Tangkas river

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

A validation method development for simultaneous LC-ESI-TOF/MS analysis of some pharmaceuticals in tangkas river-Malaysia. / Al-Qaim, Fouad F.; Abdullah, Md. Pauzi; Othman, Mohamed Rozali; Latip, Jalifah; Afiq, Wan.

In: Journal of the Brazilian Chemical Society, Vol. 25, No. 2, 02.2014, p. 271-281.

Research output: Contribution to journalArticle

Al-Qaim, Fouad F. ; Abdullah, Md. Pauzi ; Othman, Mohamed Rozali ; Latip, Jalifah ; Afiq, Wan. / A validation method development for simultaneous LC-ESI-TOF/MS analysis of some pharmaceuticals in tangkas river-Malaysia. In: Journal of the Brazilian Chemical Society. 2014 ; Vol. 25, No. 2. pp. 271-281.
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