A urea biosensor from stacked sol-gel films with immobilized nile blue chromoionophore and urease enzyme

Muawia Salameh Alqasaimeh, Yook Heng Lee, Musa Ahmad

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

An optical urea biosensor was fabricated by stacking several layers of sol-gel films. The stacking of the sol-gel films allowed the immobilization of a Nile Blue chromoionophore (ETH 5294) and urease enzyme separately without the need of any chemical attachment procedure. The absorbance response of the biosensor was monitored at 550 nm, i.e. the deprotonation of the chromoionophore. This multi-layer sol-gel film format enabled higher enzyme loading in the biosensor to be achieved. The urea optical biosensor constructed from three layers of sol-gel films that contained urease demonstrated a much wider linear response range of up to 100 mM urea when compared with biosensors that constructed from 1-2 layers of films. Analysis of urea in urine samples with this optical urea biosensor yielded results similar to that determined by a spectrophotometric method using the reagent p-dimethylaminobenzaldehyde (R 2 = 0.982, n = 6). The average recovery of urea from urine samples using this urea biosensor is approximately 103%.

Original languageEnglish
Pages (from-to)2251-2262
Number of pages12
JournalSensors
Volume7
Issue number10
Publication statusPublished - Oct 2007

Fingerprint

Urease
Biosensing Techniques
Polymethyl Methacrylate
ureas
bioinstrumentation
Biosensors
Urea
Sol-gels
enzymes
Enzymes
Gels
gels
urine
Urine
Deprotonation
Motion Pictures
Nile Blue
immobilization
Immobilization
format

Keywords

  • Clinical analysis
  • Non-leaching
  • pH optode
  • Stacked sol-gel films
  • Urea optode

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

A urea biosensor from stacked sol-gel films with immobilized nile blue chromoionophore and urease enzyme. / Alqasaimeh, Muawia Salameh; Lee, Yook Heng; Ahmad, Musa.

In: Sensors, Vol. 7, No. 10, 10.2007, p. 2251-2262.

Research output: Contribution to journalArticle

Alqasaimeh, Muawia Salameh ; Lee, Yook Heng ; Ahmad, Musa. / A urea biosensor from stacked sol-gel films with immobilized nile blue chromoionophore and urease enzyme. In: Sensors. 2007 ; Vol. 7, No. 10. pp. 2251-2262.
@article{ff622ce83bb745fea44e7e2bee2abf5f,
title = "A urea biosensor from stacked sol-gel films with immobilized nile blue chromoionophore and urease enzyme",
abstract = "An optical urea biosensor was fabricated by stacking several layers of sol-gel films. The stacking of the sol-gel films allowed the immobilization of a Nile Blue chromoionophore (ETH 5294) and urease enzyme separately without the need of any chemical attachment procedure. The absorbance response of the biosensor was monitored at 550 nm, i.e. the deprotonation of the chromoionophore. This multi-layer sol-gel film format enabled higher enzyme loading in the biosensor to be achieved. The urea optical biosensor constructed from three layers of sol-gel films that contained urease demonstrated a much wider linear response range of up to 100 mM urea when compared with biosensors that constructed from 1-2 layers of films. Analysis of urea in urine samples with this optical urea biosensor yielded results similar to that determined by a spectrophotometric method using the reagent p-dimethylaminobenzaldehyde (R 2 = 0.982, n = 6). The average recovery of urea from urine samples using this urea biosensor is approximately 103{\%}.",
keywords = "Clinical analysis, Non-leaching, pH optode, Stacked sol-gel films, Urea optode",
author = "Alqasaimeh, {Muawia Salameh} and Lee, {Yook Heng} and Musa Ahmad",
year = "2007",
month = "10",
language = "English",
volume = "7",
pages = "2251--2262",
journal = "Sensors (Switzerland)",
issn = "1424-8220",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "10",

}

TY - JOUR

T1 - A urea biosensor from stacked sol-gel films with immobilized nile blue chromoionophore and urease enzyme

AU - Alqasaimeh, Muawia Salameh

AU - Lee, Yook Heng

AU - Ahmad, Musa

PY - 2007/10

Y1 - 2007/10

N2 - An optical urea biosensor was fabricated by stacking several layers of sol-gel films. The stacking of the sol-gel films allowed the immobilization of a Nile Blue chromoionophore (ETH 5294) and urease enzyme separately without the need of any chemical attachment procedure. The absorbance response of the biosensor was monitored at 550 nm, i.e. the deprotonation of the chromoionophore. This multi-layer sol-gel film format enabled higher enzyme loading in the biosensor to be achieved. The urea optical biosensor constructed from three layers of sol-gel films that contained urease demonstrated a much wider linear response range of up to 100 mM urea when compared with biosensors that constructed from 1-2 layers of films. Analysis of urea in urine samples with this optical urea biosensor yielded results similar to that determined by a spectrophotometric method using the reagent p-dimethylaminobenzaldehyde (R 2 = 0.982, n = 6). The average recovery of urea from urine samples using this urea biosensor is approximately 103%.

AB - An optical urea biosensor was fabricated by stacking several layers of sol-gel films. The stacking of the sol-gel films allowed the immobilization of a Nile Blue chromoionophore (ETH 5294) and urease enzyme separately without the need of any chemical attachment procedure. The absorbance response of the biosensor was monitored at 550 nm, i.e. the deprotonation of the chromoionophore. This multi-layer sol-gel film format enabled higher enzyme loading in the biosensor to be achieved. The urea optical biosensor constructed from three layers of sol-gel films that contained urease demonstrated a much wider linear response range of up to 100 mM urea when compared with biosensors that constructed from 1-2 layers of films. Analysis of urea in urine samples with this optical urea biosensor yielded results similar to that determined by a spectrophotometric method using the reagent p-dimethylaminobenzaldehyde (R 2 = 0.982, n = 6). The average recovery of urea from urine samples using this urea biosensor is approximately 103%.

KW - Clinical analysis

KW - Non-leaching

KW - pH optode

KW - Stacked sol-gel films

KW - Urea optode

UR - http://www.scopus.com/inward/record.url?scp=36049047301&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36049047301&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:36049047301

VL - 7

SP - 2251

EP - 2262

JO - Sensors (Switzerland)

JF - Sensors (Switzerland)

SN - 1424-8220

IS - 10

ER -