A technique of optimization of microfiltration using a tunable platform

Jafar Alvankarian, Burhanuddin Yeop Majlis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The optimum efficiency of size-based filtration in microfluidic devices is highly dependent on characteristics of design, deformability of microparticles/cells, and fluid flow. The effects of filter pores and flow rate, which are the two major determining and related factors of characterization in the separation of particles and cells are investigated in this work. An elastomeric microfluidic device consisting of parallel arrays of pillars with mechanically tunable spacings is employed as an adjustable microfiltration platform. The tunable filtration system is used for finding the best conditions of separation of solid microbeads or deformable blood cells in a crossflow pillar-based method. It is demonstrated that increasing flow rate in the range of 1.0-80.0 μl min<sup>-1</sup> has an adverse effect on the device performance in terms of decreased separation efficiency of deformable blood cells. However, by tuning the gap size in the range of 2.5-7.5 μm, the selectivity of the separation is controlled from about 5.0 to 95.0% for white blood cells (WBCs) and 40.0 to 95.0% for red blood cells (RBCs). Finally, the best range of trapping and passing efficiencies of ∼70-80.0% simultaneously for WBCs and RBCs in whole blood sample is achieved at optimum gap size of ∼3.5-4.0 μm.

Original languageEnglish
Article number084011
JournalJournal of Micromechanics and Microengineering
Volume25
Issue number8
DOIs
Publication statusPublished - 1 Aug 2015

Fingerprint

Microfiltration
Blood
Cells
Microfluidics
Flow rate
Formability
Flow of fluids
Tuning

Keywords

  • blood cells
  • microfiltration
  • microfluidic
  • optimization
  • pillar-based

ASJC Scopus subject areas

  • Mechanical Engineering
  • Electrical and Electronic Engineering
  • Mechanics of Materials
  • Electronic, Optical and Magnetic Materials

Cite this

A technique of optimization of microfiltration using a tunable platform. / Alvankarian, Jafar; Yeop Majlis, Burhanuddin.

In: Journal of Micromechanics and Microengineering, Vol. 25, No. 8, 084011, 01.08.2015.

Research output: Contribution to journalArticle

@article{856bad66d34146eab67a4f146c598b14,
title = "A technique of optimization of microfiltration using a tunable platform",
abstract = "The optimum efficiency of size-based filtration in microfluidic devices is highly dependent on characteristics of design, deformability of microparticles/cells, and fluid flow. The effects of filter pores and flow rate, which are the two major determining and related factors of characterization in the separation of particles and cells are investigated in this work. An elastomeric microfluidic device consisting of parallel arrays of pillars with mechanically tunable spacings is employed as an adjustable microfiltration platform. The tunable filtration system is used for finding the best conditions of separation of solid microbeads or deformable blood cells in a crossflow pillar-based method. It is demonstrated that increasing flow rate in the range of 1.0-80.0 μl min-1 has an adverse effect on the device performance in terms of decreased separation efficiency of deformable blood cells. However, by tuning the gap size in the range of 2.5-7.5 μm, the selectivity of the separation is controlled from about 5.0 to 95.0{\%} for white blood cells (WBCs) and 40.0 to 95.0{\%} for red blood cells (RBCs). Finally, the best range of trapping and passing efficiencies of ∼70-80.0{\%} simultaneously for WBCs and RBCs in whole blood sample is achieved at optimum gap size of ∼3.5-4.0 μm.",
keywords = "blood cells, microfiltration, microfluidic, optimization, pillar-based",
author = "Jafar Alvankarian and {Yeop Majlis}, Burhanuddin",
year = "2015",
month = "8",
day = "1",
doi = "10.1088/0960-1317/25/8/084011",
language = "English",
volume = "25",
journal = "Journal of Micromechanics and Microengineering",
issn = "0960-1317",
publisher = "IOP Publishing Ltd.",
number = "8",

}

TY - JOUR

T1 - A technique of optimization of microfiltration using a tunable platform

AU - Alvankarian, Jafar

AU - Yeop Majlis, Burhanuddin

PY - 2015/8/1

Y1 - 2015/8/1

N2 - The optimum efficiency of size-based filtration in microfluidic devices is highly dependent on characteristics of design, deformability of microparticles/cells, and fluid flow. The effects of filter pores and flow rate, which are the two major determining and related factors of characterization in the separation of particles and cells are investigated in this work. An elastomeric microfluidic device consisting of parallel arrays of pillars with mechanically tunable spacings is employed as an adjustable microfiltration platform. The tunable filtration system is used for finding the best conditions of separation of solid microbeads or deformable blood cells in a crossflow pillar-based method. It is demonstrated that increasing flow rate in the range of 1.0-80.0 μl min-1 has an adverse effect on the device performance in terms of decreased separation efficiency of deformable blood cells. However, by tuning the gap size in the range of 2.5-7.5 μm, the selectivity of the separation is controlled from about 5.0 to 95.0% for white blood cells (WBCs) and 40.0 to 95.0% for red blood cells (RBCs). Finally, the best range of trapping and passing efficiencies of ∼70-80.0% simultaneously for WBCs and RBCs in whole blood sample is achieved at optimum gap size of ∼3.5-4.0 μm.

AB - The optimum efficiency of size-based filtration in microfluidic devices is highly dependent on characteristics of design, deformability of microparticles/cells, and fluid flow. The effects of filter pores and flow rate, which are the two major determining and related factors of characterization in the separation of particles and cells are investigated in this work. An elastomeric microfluidic device consisting of parallel arrays of pillars with mechanically tunable spacings is employed as an adjustable microfiltration platform. The tunable filtration system is used for finding the best conditions of separation of solid microbeads or deformable blood cells in a crossflow pillar-based method. It is demonstrated that increasing flow rate in the range of 1.0-80.0 μl min-1 has an adverse effect on the device performance in terms of decreased separation efficiency of deformable blood cells. However, by tuning the gap size in the range of 2.5-7.5 μm, the selectivity of the separation is controlled from about 5.0 to 95.0% for white blood cells (WBCs) and 40.0 to 95.0% for red blood cells (RBCs). Finally, the best range of trapping and passing efficiencies of ∼70-80.0% simultaneously for WBCs and RBCs in whole blood sample is achieved at optimum gap size of ∼3.5-4.0 μm.

KW - blood cells

KW - microfiltration

KW - microfluidic

KW - optimization

KW - pillar-based

UR - http://www.scopus.com/inward/record.url?scp=84938152591&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938152591&partnerID=8YFLogxK

U2 - 10.1088/0960-1317/25/8/084011

DO - 10.1088/0960-1317/25/8/084011

M3 - Article

AN - SCOPUS:84938152591

VL - 25

JO - Journal of Micromechanics and Microengineering

JF - Journal of Micromechanics and Microengineering

SN - 0960-1317

IS - 8

M1 - 084011

ER -