A survey of genes in Eimeria tenella merozoites by EST sequencing

Kiew Lian Wan, Saw Peng Chong, Szu Ting Ng, Martin W. Shirley, Fiona M.Tomley, Mohd Sanusi Jangi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A study of about 500 expressed sequence tags (ESTs), derived from a merozoite cDNA library, was initiated as an approach to generate a larger pool of gene information on Eimeria tenella. Of the ESTs, 47.7% had matches with entries in the databases, including ribosomal proteins, metabolic enzymes and proteins with other functions, of which 14.3% represented previously known E. tenella genes. Thus over 50% of the ESTs had no significant database matches. The E. tenella EST dataset contained a range of highly abundant genes comparable with that found in the EST dataset of T. gondii and may thus reflect the importance of such molecules in the biology of the apicomplexan organisms. However, comparison of the two datasets revealed very few homologies between sequences of apical organelle molecules, and provides evidence for sequence divergence between these closely-related parasites. The data presented underpin the potential value of the EST strategy for the discovery of novel genes and may allow for a more rapid increase in the knowledge and understanding of gene expression in the merozoite life cycle stage of Eimeria spp. Copyright (C) 1999 Australian Society for Parasitology.

Original languageEnglish
Pages (from-to)1885-1892
Number of pages8
JournalInternational Journal for Parasitology
Volume29
Issue number12
DOIs
Publication statusPublished - Dec 1999

Fingerprint

Eimeria tenella
Merozoites
Expressed Sequence Tags
Genes
Databases
Eimeria
Gene Pool
Ribosomal Proteins
Genetic Association Studies
Sequence Homology
Life Cycle Stages
Gene Library
Organelles
Surveys and Questionnaires
Parasites
Gene Expression
Enzymes

Keywords

  • Eimeria tenella
  • Expressed sequence tagmerozoite
  • Merozite

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Wan, K. L., Chong, S. P., Ng, S. T., Shirley, M. W., M.Tomley, F., & Sanusi Jangi, M. (1999). A survey of genes in Eimeria tenella merozoites by EST sequencing. International Journal for Parasitology, 29(12), 1885-1892. https://doi.org/10.1016/S0020-7519(99)00160-5

A survey of genes in Eimeria tenella merozoites by EST sequencing. / Wan, Kiew Lian; Chong, Saw Peng; Ng, Szu Ting; Shirley, Martin W.; M.Tomley, Fiona; Sanusi Jangi, Mohd.

In: International Journal for Parasitology, Vol. 29, No. 12, 12.1999, p. 1885-1892.

Research output: Contribution to journalArticle

Wan, KL, Chong, SP, Ng, ST, Shirley, MW, M.Tomley, F & Sanusi Jangi, M 1999, 'A survey of genes in Eimeria tenella merozoites by EST sequencing', International Journal for Parasitology, vol. 29, no. 12, pp. 1885-1892. https://doi.org/10.1016/S0020-7519(99)00160-5
Wan, Kiew Lian ; Chong, Saw Peng ; Ng, Szu Ting ; Shirley, Martin W. ; M.Tomley, Fiona ; Sanusi Jangi, Mohd. / A survey of genes in Eimeria tenella merozoites by EST sequencing. In: International Journal for Parasitology. 1999 ; Vol. 29, No. 12. pp. 1885-1892.
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