A study on polymorphisms of giardin gene of Giardia duodenalis (Malaysian) using DNA sequencing technique

Latifah Ibrahim, Teoh Kim Yong, Kiew Lian Wan, Rahmah Mohamed, Normaznah Yahya, Rohani Ahmad

Research output: Contribution to journalArticle

Abstract

Background: Giardia duodenalis is an intestinal parasite that causes diarrhoea and malabsorption in children. The parasite also infects AIDS patients with weak immune system. Objective: The objective of the study was to determine the polymorphisms of giardin gene of Malaysian isolates of G. duodenalis by DNA sequencing technique. Materials and methods: The study was carried out on six Malaysian isolates of G. duodenalis (110, 7304, 6304, M007, 2002 and 6307) from faeces of Orang AsIi (aborigine) patients admitted to Gombak Hospital. All the isolates were cultured axenically in TYI-S-33 medium. Primer pair LP2 and RP2 were used to amplify the giardin gene by polymerase chain reaction. This primer could detect G. duodenalis to the species levels specifically. PCR products were then sequenced. Results: A phylogeny tree was drawn using software from clustal-W (Pearson format) from EMBL-EBL. The phylogenetic analysis shows 2 clusters. Conclusion: We conclude that strain 2002 and 110 were similar to the pathogenic strain WB.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalInternational Medical Journal
Volume16
Issue number4
Publication statusPublished - Dec 2009

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Giardia lamblia
DNA Sequence Analysis
Genes
Parasites
Polymerase Chain Reaction
Phylogeny
Feces
Diarrhea
Immune System
Acquired Immunodeficiency Syndrome
Software

Keywords

  • Giardia duodenalis
  • Giardin gene
  • Polymorphisms
  • Sequencing technique

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A study on polymorphisms of giardin gene of Giardia duodenalis (Malaysian) using DNA sequencing technique. / Ibrahim, Latifah; Yong, Teoh Kim; Wan, Kiew Lian; Mohamed, Rahmah; Yahya, Normaznah; Ahmad, Rohani.

In: International Medical Journal, Vol. 16, No. 4, 12.2009, p. 283-288.

Research output: Contribution to journalArticle

Ibrahim, Latifah ; Yong, Teoh Kim ; Wan, Kiew Lian ; Mohamed, Rahmah ; Yahya, Normaznah ; Ahmad, Rohani. / A study on polymorphisms of giardin gene of Giardia duodenalis (Malaysian) using DNA sequencing technique. In: International Medical Journal. 2009 ; Vol. 16, No. 4. pp. 283-288.
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