A study on PCR-RFLP of Giardia duodenalis in Malaysia.

I. Latifah, K. Teoh, Kiew Lian Wan, Y. Normaznah, M. Rahmah

Research output: Contribution to journalArticle

Abstract

Giardia duodenalis causes diarrhoea and malabsorption. The objectives of the study were to detect local isolates of G. doudenalis by polymerase chain reaction (PCR) and to determine their restriction fragment length polymorphisms (RFLP). G. doudenalis isolated from stools of patients from Hospital Orang Asli Gombak were cultured axenically using TYI-S-33 medium with 10% foetal calf serum. The commercially designed primer-pair 432/433 was used to amplify a 0.52 kb segment known to encode the homologous cysteine-rich trophozoite surface antigen (tsp11 and tsa417). Results showed that the primer-pair 432/433 could amplify the target region of the local isolates. RFLP study on the identical isolates showed that all the restriction enzymes tested ( HindIII, ClaI, PstI and Kpn) gave a banding pattern similar to that of the WB strain a reference pathogenic strain from human. The reference pathogenic strain were commercially obtained from the American Type Culture Collection (ATCC).

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalThe Malaysian journal of pathology
Volume29
Issue number1
Publication statusPublished - Jun 2007
Externally publishedYes

Fingerprint

Giardia lamblia
Malaysia
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Trophozoites
Surface Antigens
Cysteine
Diarrhea
Enzymes
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Latifah, I., Teoh, K., Wan, K. L., Normaznah, Y., & Rahmah, M. (2007). A study on PCR-RFLP of Giardia duodenalis in Malaysia. The Malaysian journal of pathology, 29(1), 25-31.

A study on PCR-RFLP of Giardia duodenalis in Malaysia. / Latifah, I.; Teoh, K.; Wan, Kiew Lian; Normaznah, Y.; Rahmah, M.

In: The Malaysian journal of pathology, Vol. 29, No. 1, 06.2007, p. 25-31.

Research output: Contribution to journalArticle

Latifah, I, Teoh, K, Wan, KL, Normaznah, Y & Rahmah, M 2007, 'A study on PCR-RFLP of Giardia duodenalis in Malaysia.', The Malaysian journal of pathology, vol. 29, no. 1, pp. 25-31.
Latifah I, Teoh K, Wan KL, Normaznah Y, Rahmah M. A study on PCR-RFLP of Giardia duodenalis in Malaysia. The Malaysian journal of pathology. 2007 Jun;29(1):25-31.
Latifah, I. ; Teoh, K. ; Wan, Kiew Lian ; Normaznah, Y. ; Rahmah, M. / A study on PCR-RFLP of Giardia duodenalis in Malaysia. In: The Malaysian journal of pathology. 2007 ; Vol. 29, No. 1. pp. 25-31.
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