A study of rotenone from Derris roots of varies location, plant parts and types of solvent used

Saiful Irwan Zubairi, Mohamad Roji Sarmidi, Ramlan Abdul Aziz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Derris elliptica which is known locally as 'tuba' plant contains rotenone, a well-known bio-active compound that has the potential to be used as bio-pesticide. Bio-pesticide is the best-known alternative to the commercially available conventional pesticides and it is more environmental friendly. For that reason, the aim of this study is to obtain the best extraction solvent system that produces high yield of rotenone and to determine the rotenone content with respect to different locations of Derris roots in Johor, Malaysia. Rotenone was extracted using normal soaking extraction (NSE) process using three different parts of Derris plant and three types of solvent system. The types of Derris part were: (1) fine root, (2) coarse root and (3) stem. The types of solvent system were: (1) acetone 95% (v/v), (2) Chloroform 99.9% (v/v) and (3) a mixture of ethanol and water (9:1) added with oxalic acid (1 mg/ml). Next, the liquid crude extracts were filtered to remove any fine debris prior to the reverse-phase high performance liquid chromatography (RP-HPLC) analysis. The results showed that acetone 95% (v/v) was the best solvent system to extract the highest yield of rotenone (1.14% w/w) of Derris fine roots. Meanwhile, Kangkar Pulai was the best location to procure this domesticated forest Derris plants with high content of rotenone due to the fact that the location where it thrived was nearby to the river stream, shady spot and humid condition. For that reason, the yield of rotenone was highly affected by location, types of solvent and plant parts used.

Original languageEnglish
Pages (from-to)445-449
Number of pages5
JournalAdvances in Environmental Biology
Volume8
Issue number2
Publication statusPublished - 2014

Fingerprint

Derris
Rotenone
rotenone
plant anatomy
Pesticides
pesticides
pesticide
fine root
Acetone
acetone
Derris elliptica
Oxalic Acid
oxalic acid
reversed-phase liquid chromatography
Malaysia
extracts
Reverse-Phase Chromatography
Chloroform
chloroform
Complex Mixtures

Keywords

  • Bio-pesticide
  • Derris elliptica
  • Normal soaking extraction (NSE)
  • Rotenone

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

A study of rotenone from Derris roots of varies location, plant parts and types of solvent used. / Zubairi, Saiful Irwan; Sarmidi, Mohamad Roji; Aziz, Ramlan Abdul.

In: Advances in Environmental Biology, Vol. 8, No. 2, 2014, p. 445-449.

Research output: Contribution to journalArticle

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